FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077681341> ?p ?o ?g. }

• W2077681341 endingPage "686" @default.
• W2077681341 startingPage "663" @default.
• W2077681341 abstract "Enzymatic hydrolysis of acetylcholine (ACh) was determined in intact frog sartorius muscles or their homogenates. The Vmax was 29 nmol min-1 in intact muscles and 46 nmol min-1 per muscle in homogenates, and the Km was 6 and 0.2 mM, respectively. The muscle was divided into small segments, which were homogenized; the junctional cholinesterase (ChE) accounted for 60% of total enzyme activity. At low substrate concentrations the rate of hydrolysis was up to 30 times higher in homogenates than in intact muscles. This difference was greatly reduced at very high substrate concentrations. It appears that most of the ChE in intact muscle is 'occluded' to external ACh, mainly because the ChE at the edges of the synaptic cleft prevents the ACh from reaching the enzyme situated further inwards, which consequently does not contribute to its hydrolysis; homogenization makes all synaptic ChE accessible to added ACh. Incubation of sartorius muscles with collagenase caused an 80% decrease in ChE activity (determined in homogenates) of end-plate-containing parts which became similar to that in end-plate-free parts on which collagenase had little effect. Histochemistry showed that the tendon-muscle junction contained folds which were stained intensively for ChE. Diethyldimethylpyrophosphonate , neostigmine, eserine, and di-isopropyl fluorophosphonate inhibited ChE activity in this order of potency. The I50 values (i.e. the concentrations of the drugs which caused a 50% inhibition) were about 5 times higher in intact than in homogenized tissue. Neostigmine, 0.15 and 0.4 microM, increased the time constant of miniature end-plate currents 1.3- and 1.8-fold, and slowed down ChE activity of muscle homogenates by 1.4 and 2.1 times, respectively, without significantly affecting ACh hydrolysis by intact muscles. This indicates that synaptic ChE is not present in large excess. It is concluded that ChE activity measured in homogenates presents a better picture of in situ ChE activity than that measured in whole muscles especially for evaluating the effect of ChE inhibitors. A mathematical model for ChE-hindered diffusion of ACh is presented in an Appendix." @default.
• W2077681341 created "2016-06-24" @default.
• W2077681341 creator A5021801420 @default.
• W2077681341 creator A5035781296 @default.
• W2077681341 creator A5088448770 @default.
• W2077681341 date "1984-04-01" @default.
• W2077681341 modified "2023-10-16" @default.
• W2077681341 title "Acetylcholinesterase activity in intact and homogenized skeletal muscle of the frog." @default.
• W2077681341 cites W1543182153 @default.
• W2077681341 cites W158159343 @default.
• W2077681341 cites W1964800013 @default.
• W2077681341 cites W1977106724 @default.
• W2077681341 cites W1982218403 @default.
• W2077681341 cites W1985222283 @default.
• W2077681341 cites W1993640279 @default.
• W2077681341 cites W1998206153 @default.
• W2077681341 cites W2005681750 @default.
• W2077681341 cites W2011696262 @default.
• W2077681341 cites W2017301571 @default.
• W2077681341 cites W2018662775 @default.
• W2077681341 cites W2034684185 @default.
• W2077681341 cites W2036067191 @default.
• W2077681341 cites W2043371869 @default.
• W2077681341 cites W2050859687 @default.
• W2077681341 cites W2055379426 @default.
• W2077681341 cites W2067045168 @default.
• W2077681341 cites W2067351002 @default.
• W2077681341 cites W2068162118 @default.
• W2077681341 cites W2069085733 @default.
• W2077681341 cites W2070366880 @default.
• W2077681341 cites W2072441257 @default.
• W2077681341 cites W2074720648 @default.
• W2077681341 cites W2076188650 @default.
• W2077681341 cites W2083799894 @default.
• W2077681341 cites W2087651161 @default.
• W2077681341 cites W2097968226 @default.
• W2077681341 cites W2100185425 @default.
• W2077681341 cites W2105943730 @default.
• W2077681341 cites W2110747454 @default.
• W2077681341 cites W2122937190 @default.
• W2077681341 cites W2126702302 @default.
• W2077681341 cites W2159812499 @default.
• W2077681341 cites W2245667581 @default.
• W2077681341 cites W2408618676 @default.
• W2077681341 cites W2412531327 @default.
• W2077681341 cites W2417607648 @default.
• W2077681341 cites W2417715674 @default.
• W2077681341 cites W2416605057 @default.
• W2077681341 doi "https://doi.org/10.1113/jphysiol.1984.sp015180" @default.
• W2077681341 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1199361" @default.
• W2077681341 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6610744" @default.
• W2077681341 hasPublicationYear "1984" @default.
• W2077681341 type Work @default.
• W2077681341 sameAs 2077681341 @default.
• W2077681341 citedByCount "33" @default.
• W2077681341 countsByYear W20776813412013 @default.
• W2077681341 countsByYear W20776813412021 @default.
• W2077681341 crossrefType "journal-article" @default.
• W2077681341 hasAuthorship W2077681341A5021801420 @default.
• W2077681341 hasAuthorship W2077681341A5035781296 @default.
• W2077681341 hasAuthorship W2077681341A5088448770 @default.
• W2077681341 hasBestOaLocation W20776813412 @default.
• W2077681341 hasConcept C105702510 @default.
• W2077681341 hasConcept C126322002 @default.
• W2077681341 hasConcept C130217890 @default.
• W2077681341 hasConcept C134018914 @default.
• W2077681341 hasConcept C169760540 @default.
• W2077681341 hasConcept C181199279 @default.
• W2077681341 hasConcept C185592680 @default.
• W2077681341 hasConcept C18903297 @default.
• W2077681341 hasConcept C2775910092 @default.
• W2077681341 hasConcept C2776855439 @default.
• W2077681341 hasConcept C2777240379 @default.
• W2077681341 hasConcept C2777411258 @default.
• W2077681341 hasConcept C2778394429 @default.
• W2077681341 hasConcept C2778722038 @default.
• W2077681341 hasConcept C2778816929 @default.
• W2077681341 hasConcept C31896038 @default.
• W2077681341 hasConcept C55493867 @default.
• W2077681341 hasConcept C71924100 @default.
• W2077681341 hasConcept C86803240 @default.
• W2077681341 hasConceptScore W2077681341C105702510 @default.
• W2077681341 hasConceptScore W2077681341C126322002 @default.
• W2077681341 hasConceptScore W2077681341C130217890 @default.
• W2077681341 hasConceptScore W2077681341C134018914 @default.
• W2077681341 hasConceptScore W2077681341C169760540 @default.
• W2077681341 hasConceptScore W2077681341C181199279 @default.
• W2077681341 hasConceptScore W2077681341C185592680 @default.
• W2077681341 hasConceptScore W2077681341C18903297 @default.
• W2077681341 hasConceptScore W2077681341C2775910092 @default.
• W2077681341 hasConceptScore W2077681341C2776855439 @default.
• W2077681341 hasConceptScore W2077681341C2777240379 @default.
• W2077681341 hasConceptScore W2077681341C2777411258 @default.
• W2077681341 hasConceptScore W2077681341C2778394429 @default.
• W2077681341 hasConceptScore W2077681341C2778722038 @default.
• W2077681341 hasConceptScore W2077681341C2778816929 @default.
• W2077681341 hasConceptScore W2077681341C31896038 @default.
• W2077681341 hasConceptScore W2077681341C55493867 @default.

• next