Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077698935> ?p ?o ?g. }
- W2077698935 endingPage "187" @default.
- W2077698935 startingPage "180" @default.
- W2077698935 abstract "We have cloned and characterized a naturally occurring split mini-DnaE intein capable of protein trans-splicing in the cyanobacterium Synechococcus elongatus (Sel DnaE intein). Sel DnaE intein is homologous to Synechocystissp. PCC6803 (Ssp) DnaE intein and Nostoc punctiforme (Npu) DnaE intein, with a protein sequence identity of 60% for the N-terminal part of intein and 61% for the C-terminal part of intein. Our results demonstrate that the split reporters, split Renilla luciferase (Rluc) and enhanced green fluorescent protein (EGFP), can be reconstituted via Sel DnaE intein-mediated trans-splicing in mammalian cells. Based on Sel DnaE intein-mediated reconstitution of split Rluc, a human immunodeficiency virus (HIV) entry-mimicking cell-cell fusion assay was developed and validated as a useful assay for screening and pharmacologically characterizing potential HIV entry-targeting inhibitors." @default.
- W2077698935 created "2016-06-24" @default.
- W2077698935 creator A5027571105 @default.
- W2077698935 creator A5030775427 @default.
- W2077698935 creator A5048886458 @default.
- W2077698935 creator A5079105092 @default.
- W2077698935 creator A5089707622 @default.
- W2077698935 date "2010-12-01" @default.
- W2077698935 modified "2023-10-18" @default.
- W2077698935 title "Functional characterization of a naturally occurring trans-splicing intein from Synechococcus elongatus in a mammalian cell system" @default.
- W2077698935 cites W1504251114 @default.
- W2077698935 cites W1592537807 @default.
- W2077698935 cites W1595120220 @default.
- W2077698935 cites W1604555343 @default.
- W2077698935 cites W1620954807 @default.
- W2077698935 cites W1964892684 @default.
- W2077698935 cites W1968944419 @default.
- W2077698935 cites W1977120851 @default.
- W2077698935 cites W1979074409 @default.
- W2077698935 cites W1984811742 @default.
- W2077698935 cites W1990952318 @default.
- W2077698935 cites W2001684833 @default.
- W2077698935 cites W2001762703 @default.
- W2077698935 cites W2002701698 @default.
- W2077698935 cites W2007301673 @default.
- W2077698935 cites W2009777487 @default.
- W2077698935 cites W2011159612 @default.
- W2077698935 cites W2019057454 @default.
- W2077698935 cites W2022379457 @default.
- W2077698935 cites W2039943899 @default.
- W2077698935 cites W2041508914 @default.
- W2077698935 cites W2046769337 @default.
- W2077698935 cites W2047944454 @default.
- W2077698935 cites W2051893857 @default.
- W2077698935 cites W2065055005 @default.
- W2077698935 cites W2065120930 @default.
- W2077698935 cites W2067964854 @default.
- W2077698935 cites W2070375075 @default.
- W2077698935 cites W2071101400 @default.
- W2077698935 cites W2071844524 @default.
- W2077698935 cites W2074389035 @default.
- W2077698935 cites W2089471365 @default.
- W2077698935 cites W2097344008 @default.
- W2077698935 cites W2103125658 @default.
- W2077698935 cites W2124041751 @default.
- W2077698935 cites W2130511335 @default.
- W2077698935 cites W2144870003 @default.
- W2077698935 cites W2145035916 @default.
- W2077698935 cites W2152458717 @default.
- W2077698935 cites W2155691564 @default.
- W2077698935 cites W2158928488 @default.
- W2077698935 cites W2163729449 @default.
- W2077698935 cites W2165899507 @default.
- W2077698935 cites W2170501485 @default.
- W2077698935 cites W2275351935 @default.
- W2077698935 doi "https://doi.org/10.1016/j.ab.2010.08.018" @default.
- W2077698935 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20727340" @default.
- W2077698935 hasPublicationYear "2010" @default.
- W2077698935 type Work @default.
- W2077698935 sameAs 2077698935 @default.
- W2077698935 citedByCount "7" @default.
- W2077698935 countsByYear W20776989352012 @default.
- W2077698935 countsByYear W20776989352013 @default.
- W2077698935 countsByYear W20776989352016 @default.
- W2077698935 countsByYear W20776989352017 @default.
- W2077698935 countsByYear W20776989352023 @default.
- W2077698935 crossrefType "journal-article" @default.
- W2077698935 hasAuthorship W2077698935A5027571105 @default.
- W2077698935 hasAuthorship W2077698935A5030775427 @default.
- W2077698935 hasAuthorship W2077698935A5048886458 @default.
- W2077698935 hasAuthorship W2077698935A5079105092 @default.
- W2077698935 hasAuthorship W2077698935A5089707622 @default.
- W2077698935 hasConcept C104317684 @default.
- W2077698935 hasConcept C142613039 @default.
- W2077698935 hasConcept C153911025 @default.
- W2077698935 hasConcept C185592680 @default.
- W2077698935 hasConcept C2775954774 @default.
- W2077698935 hasConcept C47124935 @default.
- W2077698935 hasConcept C54458228 @default.
- W2077698935 hasConcept C55493867 @default.
- W2077698935 hasConcept C67705224 @default.
- W2077698935 hasConcept C69551769 @default.
- W2077698935 hasConcept C86803240 @default.
- W2077698935 hasConceptScore W2077698935C104317684 @default.
- W2077698935 hasConceptScore W2077698935C142613039 @default.
- W2077698935 hasConceptScore W2077698935C153911025 @default.
- W2077698935 hasConceptScore W2077698935C185592680 @default.
- W2077698935 hasConceptScore W2077698935C2775954774 @default.
- W2077698935 hasConceptScore W2077698935C47124935 @default.
- W2077698935 hasConceptScore W2077698935C54458228 @default.
- W2077698935 hasConceptScore W2077698935C55493867 @default.
- W2077698935 hasConceptScore W2077698935C67705224 @default.
- W2077698935 hasConceptScore W2077698935C69551769 @default.
- W2077698935 hasConceptScore W2077698935C86803240 @default.
- W2077698935 hasIssue "2" @default.
- W2077698935 hasLocation W20776989351 @default.
- W2077698935 hasLocation W20776989352 @default.
- W2077698935 hasOpenAccess W2077698935 @default.