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- W2077704225 abstract "Lipopolysaccharide-stimulated B cell cultures treated with phorbol-12,13-dibutyrate have been shown to down-regulate their steady state levels of μs RNA while the μm RNA remained constant. In contrast, the steady state levels of δ RNA was upregulated by the same treatment. By using mice transgenic for a rearranged μ or δ gene, it could be shown that the down-regulatory effect acts in trans on a defined DNA stretch and is independent of isotype. Thus, the positive effect on endogenous δ RNA steady-state levels must be regulated at the level of RNA processing. In addition, a DNA construct containing 1.5 kb of a ϵ promoter 5′ of, and an IgH enhancer 3′ of, a chloramphenicol acetyl transferase reporter gene could be down-regulated in trans by anti-μ or phorbol-12,13-dibutyrate treatment, showing that transcriptional control of Ig promoter elements can readily be observed in non-transformed B lymphocytes." @default.
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- W2077704225 date "1989-08-01" @default.
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- W2077704225 title "Regulation of immunoglobulin gene expression intrans by phorbol esters" @default.
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- W2077704225 doi "https://doi.org/10.1002/eji.1830190823" @default.
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