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• W2077765520 abstract "1. A plasmid for constitutive expression of the human interferon-alpha 1 (hIFN-alpha 1) gene in Escherichia coli is constructed on the basis of the cloning plasmid pBR322 using a strong synthetic promoter, synthetic ribosome binding site and a native hIFN-alpha 1 gene excised from a chromosomal clone. 2. The yield of recombinant hIFN-alpha 1 from E. coli LE392 cells transformed with the expression plasmid pJP1R9-hIFN-alpha 1 is evaluated to be 2-6 x 10(7) U/l bacterial culture for metabolic shaker and 6-8 x 10(7) U/l for fermentor." @default.
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• W2077765520 date "1989-01-01" @default.
• W2077765520 modified "2023-09-26" @default.
• W2077765520 title "Constitutive expression of a native human interferon-alpha 1 gene in Escherichia coli" @default.
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• W2077765520 doi "https://doi.org/10.1016/0020-711x(89)90230-9" @default.
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