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- W2077772022 abstract "The kinetics of 1-ethyl-3-(3′-dimethylaminopropyl)-carbodiimide interaction with carboxylic groups in low and high molecular weight compounds have been investigated for choosing the optimum conditions for enzyme immobilization on carboxylic carriers. Optimum pH values depended on the pKa of an acid. The ionic strength of the reaction mixture influenced the process of activation with carbodiimide and a decrease in reaction rate accompanied an increase in ionic strength. Optimum conditions for the coupling process with the use of carbodiimide activation are suggested. The process of fibrinolysin immobilization on the soluble copolymer of acrylic acid and acrylamide with carbodiimide as a coupling agent was also studied." @default.
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- W2077772022 date "1985-01-01" @default.
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- W2077772022 title "Optimization of reaction conditions during enzyme immobilization on soluble carboxyl-containing carriers" @default.
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- W2077772022 doi "https://doi.org/10.1016/0141-0229(85)90003-1" @default.
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