Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077772581> ?p ?o ?g. }
- W2077772581 endingPage "2507" @default.
- W2077772581 startingPage "2498" @default.
- W2077772581 abstract "Treatment of HIV-1 with nucleoside reverse transcription inhibitors leads to the emergence of resistance mutations in the reverse transcriptase (RT) gene. Resistance to 3'-azido-3'-deoxythymidine (AZT) and to a lesser extent to 2'-3'-didehydro-2'-3'-dideoxythymidine is mediated by phosphorolytic excision of the chain terminator. Wild-type RT excises AZT by pyrophosphorolysis, while thymidine-associated resistance mutations in RT (TAMs) favour ATP as the donor substrate. However, in vitro, resistant RT still uses pyrophosphate more efficiently than ATP. We performed in vitro (-) strong-stop DNA synthesis experiments, with wild-type and AZT-resistant HIV-1 RTs, in the presence of physiologically relevant pyrophosphate and/or ATP concentrations and found that in the presence of pyrophosphate, ATP and AZTTP, TAMs do not enhance in vitro (-) strong-stop DNA synthesis. We hypothesized that utilisation of ATP in vivo is driven by intrinsic low pyrophosphate concentrations within the reverse transcription complex, which could be explained by the packaging of a cellular pyrophosphatase. We showed that over-expressed flagged-pyrophosphatase was associated with HIV-1 viral-like particles. In addition, we demonstrated that when HIV-1 particles were purified in order to avoid cellular microvesicle contamination, a pyrophosphatase activity was specifically associated to them. The presence of a pyrophosphatase activity in close proximity to the reverse transcription complex is most likely advantageous to the virus, even in the absence of any drug pressure." @default.
- W2077772581 created "2016-06-24" @default.
- W2077772581 creator A5020187970 @default.
- W2077772581 creator A5029935844 @default.
- W2077772581 creator A5040390860 @default.
- W2077772581 creator A5041551895 @default.
- W2077772581 creator A5067744333 @default.
- W2077772581 creator A5081302791 @default.
- W2077772581 date "2012-12-01" @default.
- W2077772581 modified "2023-10-03" @default.
- W2077772581 title "A pyrophosphatase activity associated with purified HIV-1 particles" @default.
- W2077772581 cites W1480442757 @default.
- W2077772581 cites W1490915687 @default.
- W2077772581 cites W1520647240 @default.
- W2077772581 cites W1590989003 @default.
- W2077772581 cites W1654819533 @default.
- W2077772581 cites W1966857068 @default.
- W2077772581 cites W1967406495 @default.
- W2077772581 cites W1967672246 @default.
- W2077772581 cites W1967874363 @default.
- W2077772581 cites W1975040883 @default.
- W2077772581 cites W1977135599 @default.
- W2077772581 cites W1978127231 @default.
- W2077772581 cites W1979033897 @default.
- W2077772581 cites W1980418635 @default.
- W2077772581 cites W1981128154 @default.
- W2077772581 cites W1982391580 @default.
- W2077772581 cites W1985715022 @default.
- W2077772581 cites W1986852302 @default.
- W2077772581 cites W1988639299 @default.
- W2077772581 cites W1991492628 @default.
- W2077772581 cites W2003098831 @default.
- W2077772581 cites W2005460226 @default.
- W2077772581 cites W2010186221 @default.
- W2077772581 cites W2011144380 @default.
- W2077772581 cites W2015939102 @default.
- W2077772581 cites W2019812594 @default.
- W2077772581 cites W2026833019 @default.
- W2077772581 cites W2033978085 @default.
- W2077772581 cites W2041282028 @default.
- W2077772581 cites W2041751080 @default.
- W2077772581 cites W2044637338 @default.
- W2077772581 cites W2049501044 @default.
- W2077772581 cites W2050060032 @default.
- W2077772581 cites W2050647037 @default.
- W2077772581 cites W2051749789 @default.
- W2077772581 cites W2052200516 @default.
- W2077772581 cites W2059153096 @default.
- W2077772581 cites W2059435371 @default.
- W2077772581 cites W2059706128 @default.
- W2077772581 cites W2066257216 @default.
- W2077772581 cites W2066958408 @default.
- W2077772581 cites W2069233591 @default.
- W2077772581 cites W2072683386 @default.
- W2077772581 cites W2073166118 @default.
- W2077772581 cites W2079704545 @default.
- W2077772581 cites W2085310757 @default.
- W2077772581 cites W2086186867 @default.
- W2077772581 cites W2089387552 @default.
- W2077772581 cites W2090652656 @default.
- W2077772581 cites W2093967327 @default.
- W2077772581 cites W2098371287 @default.
- W2077772581 cites W2098890131 @default.
- W2077772581 cites W2099126598 @default.
- W2077772581 cites W2103737563 @default.
- W2077772581 cites W2109587611 @default.
- W2077772581 cites W2111750154 @default.
- W2077772581 cites W2116193414 @default.
- W2077772581 cites W2116893683 @default.
- W2077772581 cites W2118473036 @default.
- W2077772581 cites W2127052165 @default.
- W2077772581 cites W2128252589 @default.
- W2077772581 cites W2129088762 @default.
- W2077772581 cites W2130241957 @default.
- W2077772581 cites W2144598503 @default.
- W2077772581 cites W2146404328 @default.
- W2077772581 cites W2149232113 @default.
- W2077772581 cites W2151617473 @default.
- W2077772581 cites W2153333798 @default.
- W2077772581 cites W2155410309 @default.
- W2077772581 cites W2157513005 @default.
- W2077772581 cites W2167201468 @default.
- W2077772581 cites W2168910994 @default.
- W2077772581 cites W2169653376 @default.
- W2077772581 cites W2333399282 @default.
- W2077772581 cites W3120515989 @default.
- W2077772581 doi "https://doi.org/10.1016/j.biochi.2012.06.025" @default.
- W2077772581 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22766015" @default.
- W2077772581 hasPublicationYear "2012" @default.
- W2077772581 type Work @default.
- W2077772581 sameAs 2077772581 @default.
- W2077772581 citedByCount "1" @default.
- W2077772581 countsByYear W20777725812022 @default.
- W2077772581 crossrefType "journal-article" @default.
- W2077772581 hasAuthorship W2077772581A5020187970 @default.
- W2077772581 hasAuthorship W2077772581A5029935844 @default.
- W2077772581 hasAuthorship W2077772581A5040390860 @default.
- W2077772581 hasAuthorship W2077772581A5041551895 @default.