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• W2077774402 abstract "We report an effective approach to patterning cells on gold–silicon dioxide substrates with high precision, selectivity, stability, and reproducibility. This technique is based on photolithography and surface molecular engineering and requires no cell positioning or delivery devices, thus significantly reducing the potential damage to cells. The cell patterning was achieved by activating the gold regions of the substrate with functionalized thiols that covalently bind proteins onto the gold regions to guide subsequent cell adhesion while passivating the silicon dioxide background with polyethylene glycol to resist cell adhesion. Fourier transform infrared reflectance spectroscopy verified the successful immobilization of proteins on gold surfaces. Protein patterns were visualized by tagging proteins with Rhodamine fluorescent probes. Time-of-flight secondary ion mass spectrometry was used to characterize the chemistry of both the cell-adhesive and cell-resistant regions of surfaces after each key chemical reaction occurring during the molecular surface engineering. The ability of the engineered surfaces to guide cell adhesion was illustrated by differential interference contrast (DIC) reflectance microscopy. The cell patterning technique introduced in this study is compatible with micro- and photo-electronics, and may have many medical, environmental, and defense applications." @default.
• W2077774402 created "2016-06-24" @default.
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• W2077774402 date "2004-07-01" @default.
• W2077774402 modified "2023-10-18" @default.
• W2077774402 title "Guided cell patterning on gold–silicon dioxide substrates by surface molecular engineering" @default.
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• W2077774402 doi "https://doi.org/10.1016/j.biomaterials.2003.10.014" @default.
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