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• W2077804938 abstract "The developmental capacity of bovine oocytes matured and fertilized in vitro was investigated under five different culture systems (IVF-medium, BPM-G and TCM199 with bovine epithelial cells for in vitro culture; and rabbit and sheep oviducts for in vivo culture). Immature bovine oocytes were cultured for 24 h in TCM199 supplemented with 20% estrous cow serum and fresh granulosa cells. Fertilization in vitro was performed using pooled frozen-thawed semen from two bulls after swim-up and heparin treatments. Eighteen to 20 h after in vitro insemination, oocytes were transferred to rabbit or sheep oviducts, or were cultured in the three different media for 6 days. Recovery rates from the rabbit and sheep oviducts were 68.1% and 35.3%, respectively. In the oocytes recovered, the frequency of development to the late morula and blastocyst stages was not significantly different in rabbit (58258: 22.5%) and sheep (849: 16.3%) oviducts. The frequency of in vitro development was higher in TCM199 with added bovine oviduct cells (44361: 12.2%) than in either IVF-medium (5319: 1.6%) or BPM-G (1253: 0.4%). Although the developmental capacity of oocytes in vitro was significantly (P < 0.001) lower than in the rabbit (but not in the sheep) oviduct, the loss of oocytes in vivo counteracted this advantage of the oviduct system. The present results indicate that co-culture of embryos with bovine oviduct epithelial cells is an effective means of supporting the further development of bovine oocytes matured and fertilized in vitro." @default.
• W2077804938 created "2016-06-24" @default.
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• W2077804938 date "1989-02-01" @default.
• W2077804938 modified "2023-09-26" @default.
• W2077804938 title "Development to the late morula or blastocyst stage following in vitro maturation and fertilization of bovine oocytes" @default.
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• W2077804938 doi "https://doi.org/10.1016/0378-4320(89)90015-8" @default.
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