FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077808810> ?p ?o ?g. }

• W2077808810 endingPage "158" @default.
• W2077808810 startingPage "148" @default.
• W2077808810 abstract "A simple, sensitive ELISA that is performed in 96-well microtiter plates and that requires less than 90 minutes to complete was developed for HLA-DRB oligotyping. The second exon of HLA-DRB1 was amplified using an unlabeled forward primer and a biotinylated reverse primer and the PCR product was immobilized in avidin-coated wells. Subsequent treatment included exposure to 0.4 N NaOH to remove the nonbiotinylated sense strand, addition of a fluorescein-labeled oligonucleotide probe, one or more 5-minute stringency washes, addition of an alkaline-phosphatase-labeled anti-FL FAB, and then alkaline-phosphatase substrate and amplifier. An intense red-violet color developed within 15 minutes in positive wells and could be quantitated by OD readings at 490-495 nm. To control for stringency and to establish threshold OD values for positive reactions, biotin-labeled antisense oligos that were complementary to the probe or that differed by one or more bases were immobilized in wells in place of PCR products. The assay was sensitive to < 0.05 pmol (approximately 4 ng)/well and required only standard incubators and waterbaths and an optional microplate reader. All reagents were commercially available. The method should facilitate oligotyping of both class I and class II alleles and is adaptable for analysis of other polymorphic gene products." @default.
• W2077808810 created "2016-06-24" @default.
• W2077808810 creator A5009361679 @default.
• W2077808810 creator A5017557589 @default.
• W2077808810 creator A5061327823 @default.
• W2077808810 creator A5063106539 @default.
• W2077808810 creator A5079069182 @default.
• W2077808810 creator A5084101900 @default.
• W2077808810 date "1993-10-01" @default.
• W2077808810 modified "2023-09-27" @default.
• W2077808810 title "Rapid HLA-DR oligotyping by an enzyme-linked immunosorbent assay performed in microtiter trays" @default.
• W2077808810 cites W1517480469 @default.
• W2077808810 cites W1549464049 @default.
• W2077808810 cites W1975126775 @default.
• W2077808810 cites W1979557488 @default.
• W2077808810 cites W1979783059 @default.
• W2077808810 cites W1993027018 @default.
• W2077808810 cites W1997521650 @default.
• W2077808810 cites W1998531275 @default.
• W2077808810 cites W2015600683 @default.
• W2077808810 cites W2019257033 @default.
• W2077808810 cites W2032118018 @default.
• W2077808810 cites W2040161373 @default.
• W2077808810 cites W2054333657 @default.
• W2077808810 cites W2067229165 @default.
• W2077808810 cites W2071969793 @default.
• W2077808810 cites W2075041209 @default.
• W2077808810 cites W2077987810 @default.
• W2077808810 cites W2078298372 @default.
• W2077808810 cites W2079490247 @default.
• W2077808810 cites W2082568578 @default.
• W2077808810 cites W2085831528 @default.
• W2077808810 cites W2092636293 @default.
• W2077808810 cites W2099028937 @default.
• W2077808810 cites W2103633828 @default.
• W2077808810 cites W2108593514 @default.
• W2077808810 cites W2125909639 @default.
• W2077808810 cites W2129120773 @default.
• W2077808810 cites W2162041149 @default.
• W2077808810 cites W2163521262 @default.
• W2077808810 cites W2175410490 @default.
• W2077808810 cites W4323966460 @default.
• W2077808810 doi "https://doi.org/10.1016/0198-8859(93)90532-6" @default.
• W2077808810 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8106269" @default.
• W2077808810 hasPublicationYear "1993" @default.
• W2077808810 type Work @default.
• W2077808810 sameAs 2077808810 @default.
• W2077808810 citedByCount "22" @default.
• W2077808810 countsByYear W20778088102012 @default.
• W2077808810 countsByYear W20778088102013 @default.
• W2077808810 countsByYear W20778088102015 @default.
• W2077808810 crossrefType "journal-article" @default.
• W2077808810 hasAuthorship W2077808810A5009361679 @default.
• W2077808810 hasAuthorship W2077808810A5017557589 @default.
• W2077808810 hasAuthorship W2077808810A5061327823 @default.
• W2077808810 hasAuthorship W2077808810A5063106539 @default.
• W2077808810 hasAuthorship W2077808810A5079069182 @default.
• W2077808810 hasAuthorship W2077808810A5084101900 @default.
• W2077808810 hasConcept C111824103 @default.
• W2077808810 hasConcept C129312508 @default.
• W2077808810 hasConcept C147789679 @default.
• W2077808810 hasConcept C153911025 @default.
• W2077808810 hasConcept C160160445 @default.
• W2077808810 hasConcept C178790620 @default.
• W2077808810 hasConcept C181199279 @default.
• W2077808810 hasConcept C185592680 @default.
• W2077808810 hasConcept C204628709 @default.
• W2077808810 hasConcept C2777563447 @default.
• W2077808810 hasConcept C2778810118 @default.
• W2077808810 hasConcept C40875361 @default.
• W2077808810 hasConcept C43617362 @default.
• W2077808810 hasConcept C552990157 @default.
• W2077808810 hasConcept C55493867 @default.
• W2077808810 hasConcept C86803240 @default.
• W2077808810 hasConceptScore W2077808810C111824103 @default.
• W2077808810 hasConceptScore W2077808810C129312508 @default.
• W2077808810 hasConceptScore W2077808810C147789679 @default.
• W2077808810 hasConceptScore W2077808810C153911025 @default.
• W2077808810 hasConceptScore W2077808810C160160445 @default.
• W2077808810 hasConceptScore W2077808810C178790620 @default.
• W2077808810 hasConceptScore W2077808810C181199279 @default.
• W2077808810 hasConceptScore W2077808810C185592680 @default.
• W2077808810 hasConceptScore W2077808810C204628709 @default.
• W2077808810 hasConceptScore W2077808810C2777563447 @default.
• W2077808810 hasConceptScore W2077808810C2778810118 @default.
• W2077808810 hasConceptScore W2077808810C40875361 @default.
• W2077808810 hasConceptScore W2077808810C43617362 @default.
• W2077808810 hasConceptScore W2077808810C552990157 @default.
• W2077808810 hasConceptScore W2077808810C55493867 @default.
• W2077808810 hasConceptScore W2077808810C86803240 @default.
• W2077808810 hasIssue "2" @default.
• W2077808810 hasLocation W20778088101 @default.
• W2077808810 hasLocation W20778088102 @default.
• W2077808810 hasOpenAccess W2077808810 @default.
• W2077808810 hasPrimaryLocation W20778088101 @default.
• W2077808810 hasRelatedWork W1550018201 @default.
• W2077808810 hasRelatedWork W1970008822 @default.
• W2077808810 hasRelatedWork W1981183975 @default.

• next