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- W2077812871 abstract "QU13, a monoclonal antibody (mAb) raised against midgut (GM) antigens from Boophilus microplus and shown to recognise antigens which protec cattle from tick challenge was used to immunise cattle and rabbits to produce anti-idiotypic antibodies (AIA). Polyclonal antisera against mAb QU13 were produced in rabbits and cattle. AIA were purified from these antisera by affinity chromatography procedures. These purified AIA were found to block mAb QU13 binding to GM in enzyme-linked immunosorbent assay (ELISA). AIA purified from bovine antiserum elicited an immune response in cattle to antigens extracted by detergent from the midgut of B. microplus (TXGM) after the fourth vaccination P = 0.06)_compared with the bovine immunoglobulin (Ig) control. The mean antibody level in the group of experimental cattle vaccinated with AIA purified from rabbit antisera was significantly higher (P < 0.03) than that of bovine Ig control cattle after the fourth vaccination and an anamnestic response (P < 0.11) occurred in the rabbit AIA vaccinated group of cattle when a single booster dose of 300 μg of TXGM was given after the first tick challenge. The positive control group of cattle vaccinated with TXGM were significantly protected (P < 0.05) against tick infestation after the booster dose of 300 μg of TXGM. The AIA vaccinated groups of cattle were not protected against challenge with 20 000 larval ticks either before or after the booster injection of 300 μg of TXGM." @default.
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- W2077812871 date "1995-02-01" @default.
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- W2077812871 title "Anti-idiotypic antibodies as surrogate antigens for vaccinating against the cattle tick, Boophilus microplus" @default.
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- W2077812871 doi "https://doi.org/10.1016/0304-4017(94)00683-4" @default.
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