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• W2077819063 abstract "Abstract Purpose To examine the expression of interstitial extracellular matrix components and their role during retinal development. Material and methods Fibronectin (FN), collagen IV (Coll IV) and laminin 5 (Lam 5) expression in rat retinas from developmental stages E17 to adult were studied. In addition, PN5 full‐thickness retinas were cultured for 7 days with dispase, which selectively cleaves FN and Coll IV, at either 0.5 U/ml or 5.0 U/ml for 3 or 24 h. Eyecups and retinal cultures were examined morphologically using hematoxylin and eosin staining and immunohistochemistry. Results Coll IV, Lam 5 and FN were all transiently expressed in the interstitial matrix of the retinal layers during development. The retinal layers in dispase treated explants was severely disturbed in a dose and time dependent manner. Conclusions FN, Lam 5 and Coll IV, are present in the interstitial extracellular matrix during rat retinal development. Enzymatic cleavage of FN and Coll IV early in the lamination process disrupts the retinal layers implicating their pivotal role in this process." @default.
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• W2077819063 date "2015-03-07" @default.
• W2077819063 modified "2023-09-27" @default.
• W2077819063 title "Scaffolding the retina: The interstitial extracellular matrix during rat retinal development" @default.
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• W2077819063 doi "https://doi.org/10.1016/j.ijdevneu.2015.03.002" @default.
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