Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077819953> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2077819953 abstract "Inhibition of polypeptide chain initiation in rabbit reticulocyte lysate by phosphorylation of eukaryotic initiation factor-2(alpha) results, secondarily, in the enzymatic deacylation of Met-tRNAf on the 48 S initiation complexes that accumulate. We have prepared an antibody to a highly purified preparation of the major Met-tRNAf deacylase activity on rabbit reticulocyte ribosomes, termed deacylase II. Antibody, but not similarly purified normal IgG, completely neutralizes the activity of Met-tRNAf deacylase II and has no effect on Met-tRNAf deacylase I, a separate, minor, reticulocyte activity with the same substrate specificity but very different physical and enzymatic properties, strongly suggesting that deacylase I and II are distinct proteins. We partially purified Met-tRNAf deacylase activities from rabbit liver, myocardium and bone marrow ribosomes and found them to be similar to each other and to reticulocyte deacylase I in their enzymatic properties and insensitivity to anti-deacylase II, suggesting that deacylase I may be a general form of this enzyme, present in many cells, while deacylase II may be induced specifically during erythroid differentiation. Addition of the antibody to reticulocyte lysate incubated in the absence of hemin or presence of hemin plus 0.1 microgram/ml poly(I X C) did not reverse the inhibition of protein synthesis but did reduce the rate of turnover/utilization of Met-tRNAf and increase the level of Met-tRNAf bound to 48 S initiation complexes, demonstrating that the deacylase does not directly inhibit protein synthesis under these conditions but does mediate the deacylation, loss, and thus greater than expected turnover of Met-tRNAf in the 48 S complexes that accumulate." @default.
- W2077819953 created "2016-06-24" @default.
- W2077819953 creator A5021721465 @default.
- W2077819953 creator A5040038511 @default.
- W2077819953 creator A5068295720 @default.
- W2077819953 creator A5081276877 @default.
- W2077819953 date "1986-08-01" @default.
- W2077819953 modified "2023-09-27" @default.
- W2077819953 title "Use of an antibody to characterize and determine the role of the major Met-tRNAf deacylase from rabbit reticulocyte ribosomes" @default.
- W2077819953 cites W113011105 @default.
- W2077819953 cites W1483444054 @default.
- W2077819953 cites W1491237609 @default.
- W2077819953 cites W1547921937 @default.
- W2077819953 cites W1574158932 @default.
- W2077819953 cites W1581060680 @default.
- W2077819953 cites W1584085659 @default.
- W2077819953 cites W17120247 @default.
- W2077819953 cites W1968719690 @default.
- W2077819953 cites W1970529107 @default.
- W2077819953 cites W1978571734 @default.
- W2077819953 cites W2008698899 @default.
- W2077819953 cites W2016407154 @default.
- W2077819953 cites W2042668209 @default.
- W2077819953 cites W2059501476 @default.
- W2077819953 cites W2062417484 @default.
- W2077819953 cites W2065391770 @default.
- W2077819953 cites W2069688277 @default.
- W2077819953 cites W2075372405 @default.
- W2077819953 cites W2088987839 @default.
- W2077819953 cites W2092031513 @default.
- W2077819953 cites W2396626131 @default.
- W2077819953 cites W72118176 @default.
- W2077819953 doi "https://doi.org/10.1016/0167-4781(86)90037-0" @default.
- W2077819953 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3527271" @default.
- W2077819953 hasPublicationYear "1986" @default.
- W2077819953 type Work @default.
- W2077819953 sameAs 2077819953 @default.
- W2077819953 citedByCount "2" @default.
- W2077819953 crossrefType "journal-article" @default.
- W2077819953 hasAuthorship W2077819953A5021721465 @default.
- W2077819953 hasAuthorship W2077819953A5040038511 @default.
- W2077819953 hasAuthorship W2077819953A5068295720 @default.
- W2077819953 hasAuthorship W2077819953A5081276877 @default.
- W2077819953 hasConcept C104317684 @default.
- W2077819953 hasConcept C105580179 @default.
- W2077819953 hasConcept C153911025 @default.
- W2077819953 hasConcept C181199279 @default.
- W2077819953 hasConcept C185592680 @default.
- W2077819953 hasConcept C2776217839 @default.
- W2077819953 hasConcept C2777723881 @default.
- W2077819953 hasConcept C2778403015 @default.
- W2077819953 hasConcept C3675279 @default.
- W2077819953 hasConcept C55493867 @default.
- W2077819953 hasConcept C57409179 @default.
- W2077819953 hasConcept C67705224 @default.
- W2077819953 hasConcept C86803240 @default.
- W2077819953 hasConcept C88478588 @default.
- W2077819953 hasConceptScore W2077819953C104317684 @default.
- W2077819953 hasConceptScore W2077819953C105580179 @default.
- W2077819953 hasConceptScore W2077819953C153911025 @default.
- W2077819953 hasConceptScore W2077819953C181199279 @default.
- W2077819953 hasConceptScore W2077819953C185592680 @default.
- W2077819953 hasConceptScore W2077819953C2776217839 @default.
- W2077819953 hasConceptScore W2077819953C2777723881 @default.
- W2077819953 hasConceptScore W2077819953C2778403015 @default.
- W2077819953 hasConceptScore W2077819953C3675279 @default.
- W2077819953 hasConceptScore W2077819953C55493867 @default.
- W2077819953 hasConceptScore W2077819953C57409179 @default.
- W2077819953 hasConceptScore W2077819953C67705224 @default.
- W2077819953 hasConceptScore W2077819953C86803240 @default.
- W2077819953 hasConceptScore W2077819953C88478588 @default.
- W2077819953 hasLocation W20778199531 @default.
- W2077819953 hasLocation W20778199532 @default.
- W2077819953 hasOpenAccess W2077819953 @default.
- W2077819953 hasPrimaryLocation W20778199531 @default.
- W2077819953 hasRelatedWork W1448760128 @default.
- W2077819953 hasRelatedWork W1598799083 @default.
- W2077819953 hasRelatedWork W2029677416 @default.
- W2077819953 hasRelatedWork W2035744339 @default.
- W2077819953 hasRelatedWork W2035754702 @default.
- W2077819953 hasRelatedWork W2057272748 @default.
- W2077819953 hasRelatedWork W2057871509 @default.
- W2077819953 hasRelatedWork W2065391770 @default.
- W2077819953 hasRelatedWork W2074148999 @default.
- W2077819953 hasRelatedWork W2077819953 @default.
- W2077819953 isParatext "false" @default.
- W2077819953 isRetracted "false" @default.
- W2077819953 magId "2077819953" @default.
- W2077819953 workType "article" @default.