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- W2077851331 abstract "<h3>To the Editor.—</h3> Neutrophils of patients with Down's syndrome are known to have numerous abnormalities associated with diminished resistance to infection.<sup>1,2</sup>Several granulocyte functions have been measured in these patients: chemotaxis, phagocytosis, intracellular killing and metabolism; however, the survival of circulating neutrophils has not been investigated. The purpose of our study was to determine whether or not dead granulocytes are present in patients with trisomy 21 and in normal subjects. A supravital staining, the eosin ion exclusion test of Hanks and Wallace<sup>3</sup>as described elsewhere,<sup>4</sup>was employed in this investigation. Loopspots of 1% aqueous eosin allowed to dry on a clean slide are rehydrated with 2-mm loops of heparinized fingertip or venous blood no more than 30 minutes after the removal. After two minutes at room temperature, the percentage of granulocytes containing eosin was determined by microscopic observation of droplets that have been fixed in 95% alcohol." @default.
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- W2077851331 date "1978-08-25" @default.
- W2077851331 modified "2023-10-15" @default.
- W2077851331 title "Neutrophil Viability in Down's Syndrome" @default.
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- W2077851331 doi "https://doi.org/10.1001/jama.1978.03290080027007" @default.
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