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- W2077855397 abstract "A procedure for quantitatively binding covalently closed circular DNA to nitrocellulose is detailed. The procedure involves making DNA saturated with respect to Nal at 25°C, denaturing the DNA by heating to 80–100°C, then passing the DNA-Nal solution through a nitrocellulose membrane at 50–60°C. Form i DNA remains denatured throughout the loading procedure and is quantitatively deposited on the membrane as denatured DNA. The mechanism of binding DNA to nitrocellulose in Nal appears to be different from the binding promoted by NaCl." @default.
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- W2077855397 date "1983-03-01" @default.
- W2077855397 modified "2023-09-25" @default.
- W2077855397 title "Quantitative binding of covalently closed circular DNA to nitrocellulose in NaI" @default.
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- W2077855397 doi "https://doi.org/10.1016/0003-2697(83)90562-6" @default.
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