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• W2077855866 abstract "Summary: Phage 34 transduces some R factors to Proteus mirabilis strain 13. These trans-ductants can transmit the R factor by conjugation. Transduction frequencies vary from 1 × 10-5/plaque-forming unit (p.f.u.) adsorbed (higher than chromosomal marker transduction) to 5 × 10-8/p.f.u. adsorbed. Transduced drug resistance markers of other R factors were detected only in the presence of resident fi- factors in recipients and recombination between the R factors concerned could account for the fact that these transductants were able to transmit hybrid R factors by conjugation. Transduction of portions of R factors, including an fi+ factor occurred and some transductants were unable to transmit transduced markers by cell-to-cell contact despite the presence of resident fi-R factors. In transductants which were conjugally infectious the transduced R factor did not appear to be associated with the genome of the phage. Incompatibility had no demonstrable effect on transduction rates. The presence of an fi- resident factor reduced transduction rates when these were measurable in the absence of the resident. In two cases which involved N group resident plasmids this reduction was possibly due to restriction and host modification of transduced R factor DNA. Because of the natural resistance of the P. rettgeri host only R factors which bore the marker for kanamycin resistance could be selected in transductions with phage 7·R49. Only a T group factor was transduced. The transduction frequency was similar to that of a chromosomal marker but transductants were non-infectious." @default.
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• W2077855866 date "1973-06-01" @default.
• W2077855866 modified "2023-09-26" @default.
• W2077855866 title "Transduction of R Factors in Proteus mirabilis and P. rettgeri" @default.
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• W2077855866 doi "https://doi.org/10.1099/00221287-76-2-355" @default.
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