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- W2077856851 abstract "Although the virus of encephalitis of St. Louis type has been successfully cultivated by Maitland technic and on the chorio-allantoic membrane of developing chick1-4 the titer obtained in these cultures has been invariably low. None of the cultures showed a titer as high as 10-6 even after repeated passages. Recently Kawakita5 has reported successful cultivation of Japanese encephalitis virus in a special medium containing chick allantoic fluid. It has occurred to us to attempt to cultivate the virus of St. Louis type on the agar-tissue medium which has been found to be satisfactory for the growth of various types of rickettsiae.6, 7 The results of such a study together with a preliminary study on the immunizing value of vaccine prepared from these cultures are hereby communicated.The virus, Webster No. 3. which has full virulence for mice, was propagated in the following fashion: Infected mouse brain, diluted with serum-Tyrode solution mixture (1:5) to 10% by weight was filtered through Seitz E. K. pad a..." @default.
- W2077856851 created "2016-06-24" @default.
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- W2077856851 date "1940-04-01" @default.
- W2077856851 modified "2023-09-27" @default.
- W2077856851 title "Cultivation of Virus of Encephalitis (St. Louis Type) on Agar Tissue Medium" @default.
- W2077856851 doi "https://doi.org/10.3181/00379727-43-11326p" @default.
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