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• W2077857587 startingPage "2550" @default.
• W2077857587 abstract "An indirect competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) for the determination of dexamethasone (DEX) was developed using soybean peroxidase (SbP) as an enzyme label. A mixture of 3-(10′-phenothiazinyl)-propane-1-sulfonate (SPTZ) and 4-morpholinopyridine (MORPH) was used as an enhancer of SbP-induced chemiluminescence. Varying the concentrations of the capture antigen (DEX-ovalbumin) and specific anti-DEX antibody, the conditions of the assay were optimized. The values of IC10, IC50 and working range (IC20–IC80) of the CL-ELISA of DEX were 0.02, 0.9, 0.08–9.3 ng mL−1, respectively. It was shown that a pretreatment of cow milk samples by centrifugation and 25% methanol prevented the matrix effect of whole milk. The coefficient of variation (CV) and recovery values from the spiked milk samples estimated by the developed CL-ELISA were in the range of 2.2 to 9.9% and 82 to 142%, respectively." @default.
• W2077857587 created "2016-06-24" @default.
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• W2077857587 date "2012-01-01" @default.
• W2077857587 modified "2023-09-26" @default.
• W2077857587 title "Development of a chemiluminescent enzyme immunoassay for the determination of dexamethasone in milk" @default.
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• W2077857587 doi "https://doi.org/10.1039/c2ay25278c" @default.
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