Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077875018> ?p ?o ?g. }
- W2077875018 endingPage "313" @default.
- W2077875018 startingPage "305" @default.
- W2077875018 abstract "Campylobacter jejuni is a frequent cause of bacterial gastroenteritis in humans all over the world. Several molecular typing methods are used to study the epidemiology of Campylobacter spp. infections. The aim of the present study was to investigate the application of single-strand conformation polymorphism (SSCP) and denaturing gradient gel electrophoresis (DGGE) analysis as rapid primary subtyping methods for C. jejuni. A variable fragment from the 3' end of the flaA to the 3' end of the intergenic region, separating the flaA and flaB genes, was subjected to SSCP and DGGE analysis. A total of 48 clinical C. jejuni isolates, 49 C. jejuni strains isolated from poultry, 2 strains isolated from ducks and 1 strain isolated from a pheasant were assigned to 24 distinct SSCP patterns. Sequence analysis of the respective DNA fragments revealed that every different fla sequence type could be distinguished by SSCP. DGGE proved to be equally discriminatory. Both methods can be applied as primary subtyping methods, because pulsed-field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP) analysis further differentiated isolates belonging to the same fla sequence types." @default.
- W2077875018 created "2016-06-24" @default.
- W2077875018 creator A5005315734 @default.
- W2077875018 creator A5020842159 @default.
- W2077875018 creator A5024407452 @default.
- W2077875018 creator A5048669599 @default.
- W2077875018 date "2003-03-01" @default.
- W2077875018 modified "2023-10-16" @default.
- W2077875018 title "Application of single-strand conformation polymorphism and denaturing gradient gel electrophoresis for fla sequence typing of Campylobacter jejuni" @default.
- W2077875018 cites W1546619211 @default.
- W2077875018 cites W1587600538 @default.
- W2077875018 cites W1589349144 @default.
- W2077875018 cites W1598574080 @default.
- W2077875018 cites W1678649601 @default.
- W2077875018 cites W1922286079 @default.
- W2077875018 cites W1974670959 @default.
- W2077875018 cites W1979615913 @default.
- W2077875018 cites W1982437404 @default.
- W2077875018 cites W1982705492 @default.
- W2077875018 cites W1988086636 @default.
- W2077875018 cites W1995455927 @default.
- W2077875018 cites W2009218190 @default.
- W2077875018 cites W2030195501 @default.
- W2077875018 cites W2037268137 @default.
- W2077875018 cites W2041640845 @default.
- W2077875018 cites W2053631253 @default.
- W2077875018 cites W2068914828 @default.
- W2077875018 cites W2076958534 @default.
- W2077875018 cites W2079506307 @default.
- W2077875018 cites W2094751929 @default.
- W2077875018 cites W2097925425 @default.
- W2077875018 cites W2098618245 @default.
- W2077875018 cites W2099626769 @default.
- W2077875018 cites W2103170384 @default.
- W2077875018 cites W2108995382 @default.
- W2077875018 cites W2109410623 @default.
- W2077875018 cites W2133187834 @default.
- W2077875018 cites W2140103890 @default.
- W2077875018 cites W2143500294 @default.
- W2077875018 cites W2158045246 @default.
- W2077875018 cites W2164234706 @default.
- W2077875018 cites W2431560006 @default.
- W2077875018 cites W35938215 @default.
- W2077875018 cites W67603148 @default.
- W2077875018 doi "https://doi.org/10.1016/s0167-7012(02)00183-5" @default.
- W2077875018 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12531499" @default.
- W2077875018 hasPublicationYear "2003" @default.
- W2077875018 type Work @default.
- W2077875018 sameAs 2077875018 @default.
- W2077875018 citedByCount "15" @default.
- W2077875018 countsByYear W20778750182012 @default.
- W2077875018 countsByYear W20778750182014 @default.
- W2077875018 countsByYear W20778750182016 @default.
- W2077875018 crossrefType "journal-article" @default.
- W2077875018 hasAuthorship W2077875018A5005315734 @default.
- W2077875018 hasAuthorship W2077875018A5020842159 @default.
- W2077875018 hasAuthorship W2077875018A5024407452 @default.
- W2077875018 hasAuthorship W2077875018A5048669599 @default.
- W2077875018 hasConcept C104317684 @default.
- W2077875018 hasConcept C116084860 @default.
- W2077875018 hasConcept C129219603 @default.
- W2077875018 hasConcept C135763542 @default.
- W2077875018 hasConcept C144024400 @default.
- W2077875018 hasConcept C149923435 @default.
- W2077875018 hasConcept C153523973 @default.
- W2077875018 hasConcept C198549448 @default.
- W2077875018 hasConcept C199360897 @default.
- W2077875018 hasConcept C2776957140 @default.
- W2077875018 hasConcept C2779910956 @default.
- W2077875018 hasConcept C2781209916 @default.
- W2077875018 hasConcept C2908647359 @default.
- W2077875018 hasConcept C41008148 @default.
- W2077875018 hasConcept C42062724 @default.
- W2077875018 hasConcept C42084846 @default.
- W2077875018 hasConcept C49105822 @default.
- W2077875018 hasConcept C523546767 @default.
- W2077875018 hasConcept C54355233 @default.
- W2077875018 hasConcept C61053724 @default.
- W2077875018 hasConcept C81977670 @default.
- W2077875018 hasConcept C83852419 @default.
- W2077875018 hasConcept C86803240 @default.
- W2077875018 hasConcept C89423630 @default.
- W2077875018 hasConceptScore W2077875018C104317684 @default.
- W2077875018 hasConceptScore W2077875018C116084860 @default.
- W2077875018 hasConceptScore W2077875018C129219603 @default.
- W2077875018 hasConceptScore W2077875018C135763542 @default.
- W2077875018 hasConceptScore W2077875018C144024400 @default.
- W2077875018 hasConceptScore W2077875018C149923435 @default.
- W2077875018 hasConceptScore W2077875018C153523973 @default.
- W2077875018 hasConceptScore W2077875018C198549448 @default.
- W2077875018 hasConceptScore W2077875018C199360897 @default.
- W2077875018 hasConceptScore W2077875018C2776957140 @default.
- W2077875018 hasConceptScore W2077875018C2779910956 @default.
- W2077875018 hasConceptScore W2077875018C2781209916 @default.
- W2077875018 hasConceptScore W2077875018C2908647359 @default.
- W2077875018 hasConceptScore W2077875018C41008148 @default.
- W2077875018 hasConceptScore W2077875018C42062724 @default.
- W2077875018 hasConceptScore W2077875018C42084846 @default.