FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077883207> ?p ?o ?g. }

• W2077883207 endingPage "450" @default.
• W2077883207 startingPage "435" @default.
• W2077883207 abstract "Abstract A comprehensive characterization of the enzyme kinetics and stability is required in order to design biotransformation processes efficiently. In particular, the characterization of the operational stability (stability under process conditions) of biocatalysts lacks established procedures and the few reported procedures have hardly been evaluated by transfer to reactor scale. In the scope of this modeling study three potentially applicable characterization methods were tested: (i) progress curves recorded at different temperatures; (ii) isothermal continuous reactor operation at different (elevated) temperatures; and (iii) continuous reactor operation with a temperature gradient. Input data from those procedures were generated in-silico by introducing two virtual dimeric enzymes with defined, complex deactivation mechanisms. The applied degradation pathways account for experimentally proven phenomena, which were neglected in operational stability descriptions so far, such as multiple intermediate unfolding state and subunit dissociation. By introducing a random normal error data sets with typical experimental variance were generated. On the basis of these data a model-based experimental analysis for parameter estimation was applied which included the testing of different simplified models (with increasing complexity until over-parameterization became evident). The obtained fits were evaluated by the lack of fit variance and the significance level of the F-statistics. Both criteria indicated a dimeric model with two intermediate unfolding states as best description, which was the model with the highest similarity to the models that had been applied to produce the data in the first place. When subjecting the obtained parameterization to a virtual process design task the quality of the prediction could be easily tested by comparison with the prediction from the original mechanistic model that was used to produce the input data. The obtained results clearly indicate that the often used Lumry-Eyring type models should not be used as empiric description for potentially complex multimeric enzyme deactivation mechanisms as it is done in current operational stability investigations. Next, for the continuous reactor procedures a nice correlation between the obtained significance level of the F-statistics and the accuracy of process prediction was found demonstrating the usefulness of these experimental procedures for parameter estimation. Further, it was possible to estimate a specific threshold significance level that can be used as an indicator for reliable parameterizations with respect to process design." @default.
• W2077883207 created "2016-06-24" @default.
• W2077883207 creator A5031880442 @default.
• W2077883207 creator A5086235336 @default.
• W2077883207 date "2012-10-01" @default.
• W2077883207 modified "2023-09-27" @default.
• W2077883207 title "Model-based characterization of operational stability of multimeric enzymes with complex deactivation behavior: An in-silico investigation" @default.
• W2077883207 cites W1516339579 @default.
• W2077883207 cites W1540340390 @default.
• W2077883207 cites W166851970 @default.
• W2077883207 cites W1964103819 @default.
• W2077883207 cites W1971711803 @default.
• W2077883207 cites W1977877548 @default.
• W2077883207 cites W1977962237 @default.
• W2077883207 cites W1980077712 @default.
• W2077883207 cites W1980478984 @default.
• W2077883207 cites W1985941856 @default.
• W2077883207 cites W1986920361 @default.
• W2077883207 cites W1989400482 @default.
• W2077883207 cites W1992488465 @default.
• W2077883207 cites W1992695075 @default.
• W2077883207 cites W1994024442 @default.
• W2077883207 cites W1995706284 @default.
• W2077883207 cites W1995963602 @default.
• W2077883207 cites W1999832381 @default.
• W2077883207 cites W2002050205 @default.
• W2077883207 cites W2005440198 @default.
• W2077883207 cites W2008029791 @default.
• W2077883207 cites W2017064467 @default.
• W2077883207 cites W2021486592 @default.
• W2077883207 cites W2022280363 @default.
• W2077883207 cites W2026048837 @default.
• W2077883207 cites W2026894454 @default.
• W2077883207 cites W2033658542 @default.
• W2077883207 cites W2035762247 @default.
• W2077883207 cites W2040302146 @default.
• W2077883207 cites W2048363942 @default.
• W2077883207 cites W2050465327 @default.
• W2077883207 cites W2057206798 @default.
• W2077883207 cites W205739566 @default.
• W2077883207 cites W2057427542 @default.
• W2077883207 cites W2060160295 @default.
• W2077883207 cites W2061521483 @default.
• W2077883207 cites W2064641974 @default.
• W2077883207 cites W2072732989 @default.
• W2077883207 cites W2073685288 @default.
• W2077883207 cites W2075100950 @default.
• W2077883207 cites W2079257291 @default.
• W2077883207 cites W2082300538 @default.
• W2077883207 cites W2087564275 @default.
• W2077883207 cites W2097043151 @default.
• W2077883207 cites W2097985831 @default.
• W2077883207 cites W2109319929 @default.
• W2077883207 cites W2114992196 @default.
• W2077883207 cites W2121351614 @default.
• W2077883207 cites W2142731440 @default.
• W2077883207 cites W2142967403 @default.
• W2077883207 cites W2153004318 @default.
• W2077883207 cites W2159385643 @default.
• W2077883207 cites W2329952272 @default.
• W2077883207 cites W4244687418 @default.
• W2077883207 cites W4321429423 @default.
• W2077883207 doi "https://doi.org/10.1016/j.ces.2012.05.030" @default.
• W2077883207 hasPublicationYear "2012" @default.
• W2077883207 type Work @default.
• W2077883207 sameAs 2077883207 @default.
• W2077883207 citedByCount "11" @default.
• W2077883207 countsByYear W20778832072013 @default.
• W2077883207 countsByYear W20778832072014 @default.
• W2077883207 countsByYear W20778832072015 @default.
• W2077883207 countsByYear W20778832072019 @default.
• W2077883207 countsByYear W20778832072020 @default.
• W2077883207 countsByYear W20778832072022 @default.
• W2077883207 crossrefType "journal-article" @default.
• W2077883207 hasAuthorship W2077883207A5031880442 @default.
• W2077883207 hasAuthorship W2077883207A5086235336 @default.
• W2077883207 hasConcept C104317684 @default.
• W2077883207 hasConcept C112972136 @default.
• W2077883207 hasConcept C119857082 @default.
• W2077883207 hasConcept C12554922 @default.
• W2077883207 hasConcept C171250308 @default.
• W2077883207 hasConcept C181199279 @default.
• W2077883207 hasConcept C185592680 @default.
• W2077883207 hasConcept C186060115 @default.
• W2077883207 hasConcept C192562407 @default.
• W2077883207 hasConcept C2775905019 @default.
• W2077883207 hasConcept C2780841128 @default.
• W2077883207 hasConcept C41008148 @default.
• W2077883207 hasConcept C43617362 @default.
• W2077883207 hasConcept C55493867 @default.
• W2077883207 hasConcept C86803240 @default.
• W2077883207 hasConceptScore W2077883207C104317684 @default.
• W2077883207 hasConceptScore W2077883207C112972136 @default.
• W2077883207 hasConceptScore W2077883207C119857082 @default.
• W2077883207 hasConceptScore W2077883207C12554922 @default.
• W2077883207 hasConceptScore W2077883207C171250308 @default.
• W2077883207 hasConceptScore W2077883207C181199279 @default.
• W2077883207 hasConceptScore W2077883207C185592680 @default.

• next