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- W2077886680 abstract "Abstract Thymidine kinase (EC 2.7.1.75) from 36-h regenerating rat liver was purified 2600-fold relative to the specific activity of enzyme in the cytoplasmic fraction. About 8 stained bands were visible after electrophoresis on sodium dodecylsulfate-containing polyacrylamide gels. The molecular weight estimated by centrifugation through linear sucrose gradients was 69 000. K m estimates for thymidine and ATP were 3.2 μM and 0.3 mM, respectively. The enzyme was inhibited by dTTP; inhibition was non-competitive with respect to the phosphate donor and complex with respect to the phosphate acceptor." @default.
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- W2077886680 date "1974-05-01" @default.
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- W2077886680 title "Purification and properties of thymidine kinase from regenerating rat liver" @default.
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- W2077886680 doi "https://doi.org/10.1016/0005-2744(74)90217-4" @default.
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