Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077893546> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2077893546 abstract "Abstract The external and cytoplasmic surfaces of the sea urchin egg at fertilization have been examined with the scanning electron microscope (SEM). The outside events were documented by glueing eggs to polylysine coated glass plates, adding sperm and fixing rapidly. To reveal the inner aspects of the surface as the sperm travels through it to reach the egg cytoplasm, the fertilized egg surface was isolated in 0.3 M KC1, 0.35 M glycine, 2 mM MgCl 2 , 2 mM EGTA, pH 7.5, glued onto a polylysine-coated plate and processed for the SEM. The events of spermatozoon attachment, membrane fusion, sperm entry, rotation and detachment into the egg cytoplasm as well as the associated cortical changes are described. The egg cortex is revealed to be a uniform network of fibrous bundles. The spermatozoon initially attaches to the egg surface by the acrosomal filament. As membrane fusion occurs between the gametes, the plasma membrane of the egg engulfs the sperm, the cortical granules start to discharge and a spreading surface deformation, possibly caused by a cortical contraction, is initiated. The perpendicularly entering spermatozoon is surrounded by a cluster of elongate microvilli which appear to have 235 nm vesicles associated with their bases. The sperm is prevented by the cortex from directly entering the egg cytoplasm and lies upon the egg surface between the plasma membrane and the matrix of cortical fibers. It is subsequently rotated additionally to enter the egg cytoplasm with the posterior end first. A scar is left in the cortex where the spermatozoon penetrated. The egg cortex is shown to consist of 50–200 nm uniformly arranged fibers, and its thickness ranges from 0.2 to 0.5 μm. It is speculated that this structure may be contractile." @default.
- W2077893546 created "2016-06-24" @default.
- W2077893546 creator A5060338506 @default.
- W2077893546 creator A5061656670 @default.
- W2077893546 date "1976-03-15" @default.
- W2077893546 modified "2023-09-23" @default.
- W2077893546 title "The penetration of the spermatozoon through the sea urchin egg surface at fertilization *1Observations from the outside on whole eggs and from the inside on isolated surfaces" @default.
- W2077893546 cites W1963446165 @default.
- W2077893546 cites W1987291682 @default.
- W2077893546 cites W2013882419 @default.
- W2077893546 cites W2037576512 @default.
- W2077893546 cites W2041590192 @default.
- W2077893546 cites W2058442820 @default.
- W2077893546 cites W2059422383 @default.
- W2077893546 cites W2071337535 @default.
- W2077893546 cites W2122895004 @default.
- W2077893546 cites W2125384165 @default.
- W2077893546 cites W2135071188 @default.
- W2077893546 cites W2145260575 @default.
- W2077893546 cites W2797564667 @default.
- W2077893546 doi "https://doi.org/10.1016/0014-4827(76)90444-4" @default.
- W2077893546 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/946419" @default.
- W2077893546 hasPublicationYear "1976" @default.
- W2077893546 type Work @default.
- W2077893546 sameAs 2077893546 @default.
- W2077893546 citedByCount "69" @default.
- W2077893546 countsByYear W20778935462013 @default.
- W2077893546 countsByYear W20778935462019 @default.
- W2077893546 countsByYear W20778935462021 @default.
- W2077893546 crossrefType "journal-article" @default.
- W2077893546 hasAuthorship W2077893546A5060338506 @default.
- W2077893546 hasAuthorship W2077893546A5061656670 @default.
- W2077893546 hasConcept C105702510 @default.
- W2077893546 hasConcept C12554922 @default.
- W2077893546 hasConcept C130316041 @default.
- W2077893546 hasConcept C190062978 @default.
- W2077893546 hasConcept C196843134 @default.
- W2077893546 hasConcept C2776632385 @default.
- W2077893546 hasConcept C2776690073 @default.
- W2077893546 hasConcept C2776908312 @default.
- W2077893546 hasConcept C2777394357 @default.
- W2077893546 hasConcept C2781087480 @default.
- W2077893546 hasConcept C41625074 @default.
- W2077893546 hasConcept C55493867 @default.
- W2077893546 hasConcept C59822182 @default.
- W2077893546 hasConcept C86803240 @default.
- W2077893546 hasConcept C88972607 @default.
- W2077893546 hasConcept C90642696 @default.
- W2077893546 hasConcept C95444343 @default.
- W2077893546 hasConceptScore W2077893546C105702510 @default.
- W2077893546 hasConceptScore W2077893546C12554922 @default.
- W2077893546 hasConceptScore W2077893546C130316041 @default.
- W2077893546 hasConceptScore W2077893546C190062978 @default.
- W2077893546 hasConceptScore W2077893546C196843134 @default.
- W2077893546 hasConceptScore W2077893546C2776632385 @default.
- W2077893546 hasConceptScore W2077893546C2776690073 @default.
- W2077893546 hasConceptScore W2077893546C2776908312 @default.
- W2077893546 hasConceptScore W2077893546C2777394357 @default.
- W2077893546 hasConceptScore W2077893546C2781087480 @default.
- W2077893546 hasConceptScore W2077893546C41625074 @default.
- W2077893546 hasConceptScore W2077893546C55493867 @default.
- W2077893546 hasConceptScore W2077893546C59822182 @default.
- W2077893546 hasConceptScore W2077893546C86803240 @default.
- W2077893546 hasConceptScore W2077893546C88972607 @default.
- W2077893546 hasConceptScore W2077893546C90642696 @default.
- W2077893546 hasConceptScore W2077893546C95444343 @default.
- W2077893546 hasLocation W20778935461 @default.
- W2077893546 hasLocation W20778935462 @default.
- W2077893546 hasOpenAccess W2077893546 @default.
- W2077893546 hasPrimaryLocation W20778935461 @default.
- W2077893546 hasRelatedWork W1974981660 @default.
- W2077893546 hasRelatedWork W2017217331 @default.
- W2077893546 hasRelatedWork W2080090703 @default.
- W2077893546 hasRelatedWork W2087537535 @default.
- W2077893546 hasRelatedWork W2280120595 @default.
- W2077893546 hasRelatedWork W2384177213 @default.
- W2077893546 hasRelatedWork W2413808191 @default.
- W2077893546 hasRelatedWork W2474456586 @default.
- W2077893546 hasRelatedWork W2743813579 @default.
- W2077893546 hasRelatedWork W47055206 @default.
- W2077893546 isParatext "false" @default.
- W2077893546 isRetracted "false" @default.
- W2077893546 magId "2077893546" @default.
- W2077893546 workType "article" @default.