Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077997671> ?p ?o ?g. }
- W2077997671 endingPage "101" @default.
- W2077997671 startingPage "91" @default.
- W2077997671 abstract "Formic acid in high concentration is an extremely potent solvent for proteins, particularly for hydrophobic ones. 60% Formic acid, necessary for solubilization of structural polypeptides of poliovirus and other proteins, modified at the cysteines, was used together with 2-propanol or acetonitrile as organic modifier for gradient elution in reversed-phase high-performance liquid chromatography. Several reversed-phase columns were tested. In each case, polypeptides were eluted quantitatively. It was demonstrated that this solvent system, with its high proportion of formic acid, did not affect the size, hydrophobicity and charge of the separated polypeptides. By injection into rabbits of poliovirus polypeptides, obtained in high purity by chromatography in the new solvent system, monospecific antibodies were induced, the specificity of which was determined by immunoprecipitation." @default.
- W2077997671 created "2016-06-24" @default.
- W2077997671 creator A5053891027 @default.
- W2077997671 creator A5058080023 @default.
- W2077997671 date "1985-06-01" @default.
- W2077997671 modified "2023-10-17" @default.
- W2077997671 title "Characterization of a solvent system for separation of water-insoluble poliovirus proteins by reversed-phase high-performance liquid chromatography" @default.
- W2077997671 cites W1498606106 @default.
- W2077997671 cites W1570828652 @default.
- W2077997671 cites W1572064562 @default.
- W2077997671 cites W1583198424 @default.
- W2077997671 cites W1971321764 @default.
- W2077997671 cites W1972338774 @default.
- W2077997671 cites W1973953090 @default.
- W2077997671 cites W1982666542 @default.
- W2077997671 cites W1984501109 @default.
- W2077997671 cites W1986199836 @default.
- W2077997671 cites W1989096596 @default.
- W2077997671 cites W1993305231 @default.
- W2077997671 cites W1995797614 @default.
- W2077997671 cites W2002788391 @default.
- W2077997671 cites W2004187729 @default.
- W2077997671 cites W2019748889 @default.
- W2077997671 cites W2023758525 @default.
- W2077997671 cites W2034365299 @default.
- W2077997671 cites W2041584824 @default.
- W2077997671 cites W2053878015 @default.
- W2077997671 cites W2056718050 @default.
- W2077997671 cites W2058423707 @default.
- W2077997671 cites W2059173399 @default.
- W2077997671 cites W2060579461 @default.
- W2077997671 cites W2069041067 @default.
- W2077997671 cites W2080330945 @default.
- W2077997671 cites W2083985132 @default.
- W2077997671 cites W2084834562 @default.
- W2077997671 cites W2087930279 @default.
- W2077997671 cites W2088852772 @default.
- W2077997671 cites W2133469838 @default.
- W2077997671 cites W2137299077 @default.
- W2077997671 cites W2141280138 @default.
- W2077997671 cites W2155527842 @default.
- W2077997671 cites W2170055655 @default.
- W2077997671 cites W2267143376 @default.
- W2077997671 cites W2275843281 @default.
- W2077997671 cites W2413409309 @default.
- W2077997671 cites W2428503963 @default.
- W2077997671 cites W29337838 @default.
- W2077997671 cites W3026554484 @default.
- W2077997671 cites W58707237 @default.
- W2077997671 doi "https://doi.org/10.1016/s0021-9673(01)87434-3" @default.
- W2077997671 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2993331" @default.
- W2077997671 hasPublicationYear "1985" @default.
- W2077997671 type Work @default.
- W2077997671 sameAs 2077997671 @default.
- W2077997671 citedByCount "78" @default.
- W2077997671 countsByYear W20779976712012 @default.
- W2077997671 countsByYear W20779976712013 @default.
- W2077997671 countsByYear W20779976712014 @default.
- W2077997671 countsByYear W20779976712015 @default.
- W2077997671 countsByYear W20779976712016 @default.
- W2077997671 countsByYear W20779976712017 @default.
- W2077997671 countsByYear W20779976712021 @default.
- W2077997671 crossrefType "journal-article" @default.
- W2077997671 hasAuthorship W2077997671A5053891027 @default.
- W2077997671 hasAuthorship W2077997671A5058080023 @default.
- W2077997671 hasConcept C116817701 @default.
- W2077997671 hasConcept C159047783 @default.
- W2077997671 hasConcept C178790620 @default.
- W2077997671 hasConcept C179998833 @default.
- W2077997671 hasConcept C181199279 @default.
- W2077997671 hasConcept C185592680 @default.
- W2077997671 hasConcept C193363816 @default.
- W2077997671 hasConcept C2522874641 @default.
- W2077997671 hasConcept C2777379063 @default.
- W2077997671 hasConcept C2777463227 @default.
- W2077997671 hasConcept C2779631682 @default.
- W2077997671 hasConcept C2780471494 @default.
- W2077997671 hasConcept C43617362 @default.
- W2077997671 hasConcept C44280652 @default.
- W2077997671 hasConcept C45188465 @default.
- W2077997671 hasConcept C73826308 @default.
- W2077997671 hasConcept C86803240 @default.
- W2077997671 hasConceptScore W2077997671C116817701 @default.
- W2077997671 hasConceptScore W2077997671C159047783 @default.
- W2077997671 hasConceptScore W2077997671C178790620 @default.
- W2077997671 hasConceptScore W2077997671C179998833 @default.
- W2077997671 hasConceptScore W2077997671C181199279 @default.
- W2077997671 hasConceptScore W2077997671C185592680 @default.
- W2077997671 hasConceptScore W2077997671C193363816 @default.
- W2077997671 hasConceptScore W2077997671C2522874641 @default.
- W2077997671 hasConceptScore W2077997671C2777379063 @default.
- W2077997671 hasConceptScore W2077997671C2777463227 @default.
- W2077997671 hasConceptScore W2077997671C2779631682 @default.
- W2077997671 hasConceptScore W2077997671C2780471494 @default.
- W2077997671 hasConceptScore W2077997671C43617362 @default.
- W2077997671 hasConceptScore W2077997671C44280652 @default.
- W2077997671 hasConceptScore W2077997671C45188465 @default.
- W2077997671 hasConceptScore W2077997671C73826308 @default.