Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078004557> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2078004557 endingPage "10863" @default.
- W2078004557 startingPage "10854" @default.
- W2078004557 abstract "GTP-l-fucose pyrophosphorylase (GFPP, E. C. 2.7.7.30) catalyzes the reversible condensation of guanosine triphosphate and beta-l-fucose-1-phosphate to form the nucleotide-sugar GDP-beta-l-fucose. The enzyme functions primarily in the mammalian liver and kidney to salvage free l-fucose during the breakdown of glycolipids and glycoproteins. The mechanism by which this protein discriminates between substrate and nonsubstrate molecules has been elucidated for the first time in this study. The ability of GFPP to form nucleotide-sugars from a series of base-, ribose-, phosphate-, and hexose-modified precursor molecules has revealed that the enzyme active site senses a series of substrate substituents that drive substrate/nonsubstrate discrimination. These substituents alter the ability of the precursor molecule to interact with the enzyme, as measured by either changes in the Michaelis constant, K(m), the binding affinity, K(a), or through changes in enzymatic turnover, k(cat). In this work, the combined substrate binding and enzyme analysis has revealed that the nature of the purine base is the major determinant in substrate specificity, followed by the nature of the hexose-1-P, and finally by the ribose moiety. Binding is enthalpy-driven and does not involve proton transfer. For the majority of nucleotide-sugar analogues, binding to GFPP is entropically unfavorable; however, surprisingly, a few of the substrate analogues tested bind to GFPP with a favorable entropic term." @default.
- W2078004557 created "2016-06-24" @default.
- W2078004557 creator A5035989346 @default.
- W2078004557 creator A5059519406 @default.
- W2078004557 date "2005-07-19" @default.
- W2078004557 modified "2023-10-01" @default.
- W2078004557 title "Substrate Discrimination by the Human GTP Fucose Pyrophosphorylase" @default.
- W2078004557 cites W1660964108 @default.
- W2078004557 cites W1882348122 @default.
- W2078004557 cites W1971588828 @default.
- W2078004557 cites W1991717719 @default.
- W2078004557 cites W2013428743 @default.
- W2078004557 cites W2017745812 @default.
- W2078004557 cites W2040519972 @default.
- W2078004557 cites W2061164391 @default.
- W2078004557 cites W2067043700 @default.
- W2078004557 cites W2085226274 @default.
- W2078004557 cites W2113406360 @default.
- W2078004557 cites W2132112146 @default.
- W2078004557 cites W2139514746 @default.
- W2078004557 cites W2175913572 @default.
- W2078004557 cites W2400875063 @default.
- W2078004557 doi "https://doi.org/10.1021/bi0503605" @default.
- W2078004557 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16086588" @default.
- W2078004557 hasPublicationYear "2005" @default.
- W2078004557 type Work @default.
- W2078004557 sameAs 2078004557 @default.
- W2078004557 citedByCount "27" @default.
- W2078004557 countsByYear W20780045572012 @default.
- W2078004557 countsByYear W20780045572013 @default.
- W2078004557 countsByYear W20780045572014 @default.
- W2078004557 countsByYear W20780045572015 @default.
- W2078004557 countsByYear W20780045572017 @default.
- W2078004557 countsByYear W20780045572018 @default.
- W2078004557 countsByYear W20780045572019 @default.
- W2078004557 countsByYear W20780045572020 @default.
- W2078004557 countsByYear W20780045572021 @default.
- W2078004557 crossrefType "journal-article" @default.
- W2078004557 hasAuthorship W2078004557A5035989346 @default.
- W2078004557 hasAuthorship W2078004557A5059519406 @default.
- W2078004557 hasConcept C104317684 @default.
- W2078004557 hasConcept C108625454 @default.
- W2078004557 hasConcept C118716 @default.
- W2078004557 hasConcept C181199279 @default.
- W2078004557 hasConcept C185592680 @default.
- W2078004557 hasConcept C18903297 @default.
- W2078004557 hasConcept C24107716 @default.
- W2078004557 hasConcept C2776393687 @default.
- W2078004557 hasConcept C2776841590 @default.
- W2078004557 hasConcept C2777289219 @default.
- W2078004557 hasConcept C2777709666 @default.
- W2078004557 hasConcept C2779461342 @default.
- W2078004557 hasConcept C512185932 @default.
- W2078004557 hasConcept C55493867 @default.
- W2078004557 hasConcept C71240020 @default.
- W2078004557 hasConcept C86803240 @default.
- W2078004557 hasConceptScore W2078004557C104317684 @default.
- W2078004557 hasConceptScore W2078004557C108625454 @default.
- W2078004557 hasConceptScore W2078004557C118716 @default.
- W2078004557 hasConceptScore W2078004557C181199279 @default.
- W2078004557 hasConceptScore W2078004557C185592680 @default.
- W2078004557 hasConceptScore W2078004557C18903297 @default.
- W2078004557 hasConceptScore W2078004557C24107716 @default.
- W2078004557 hasConceptScore W2078004557C2776393687 @default.
- W2078004557 hasConceptScore W2078004557C2776841590 @default.
- W2078004557 hasConceptScore W2078004557C2777289219 @default.
- W2078004557 hasConceptScore W2078004557C2777709666 @default.
- W2078004557 hasConceptScore W2078004557C2779461342 @default.
- W2078004557 hasConceptScore W2078004557C512185932 @default.
- W2078004557 hasConceptScore W2078004557C55493867 @default.
- W2078004557 hasConceptScore W2078004557C71240020 @default.
- W2078004557 hasConceptScore W2078004557C86803240 @default.
- W2078004557 hasIssue "32" @default.
- W2078004557 hasLocation W20780045571 @default.
- W2078004557 hasLocation W20780045572 @default.
- W2078004557 hasOpenAccess W2078004557 @default.
- W2078004557 hasPrimaryLocation W20780045571 @default.
- W2078004557 hasRelatedWork W1972208598 @default.
- W2078004557 hasRelatedWork W1976751119 @default.
- W2078004557 hasRelatedWork W1997683263 @default.
- W2078004557 hasRelatedWork W2012367667 @default.
- W2078004557 hasRelatedWork W2046972484 @default.
- W2078004557 hasRelatedWork W2067218560 @default.
- W2078004557 hasRelatedWork W2147675827 @default.
- W2078004557 hasRelatedWork W2171154917 @default.
- W2078004557 hasRelatedWork W4384079124 @default.
- W2078004557 hasRelatedWork W979752654 @default.
- W2078004557 hasVolume "44" @default.
- W2078004557 isParatext "false" @default.
- W2078004557 isRetracted "false" @default.
- W2078004557 magId "2078004557" @default.
- W2078004557 workType "article" @default.