FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078010237> ?p ?o ?g. }

• W2078010237 abstract "To the Editor: Extraintestinal pathogenic Escherichai coli (ExPEC) bacteria have the ability to cause diverse and serious diseases, such as urinary tract infections (UTIs) and bacteremia (1–3); incidence of bacteremia is increasing globally (4). The emergence of multidrug resistance in E. coli is also becoming a global concern, with particular emphasis on E. coli sequence type (ST) 131, which is being increasingly reported in UTIs. Drug resistance is mediated by extended-spectrum β-lactamases (ESBLs), mainly of the CTX-M family, particularly CTX-M-15 and 14, and less frequently of the SHV and OXA families (5,6). Few studies are available regarding the characterization of E. coli strains causing bacteremia.We characterized 140 E. coli isolates from bacteremia patients treated at Nottingham University Hospital (Nottingham, UK) over a 5-month period, with the aim of developing an epidemiologic profile of the population of ExPEC that causes bacteremia. For context, we compared the isolates with 125 E. coli isolates from urine samples collected during the same period. Cases were selected to include isolates from a diverse patient group: patient ages ranged from 1 month to 90 years; patient sex was evenly divided between male and female; infections were community- and hospital-associated; and suspected sources of infection varied. Antimicrobial drug susceptibility tests, PCR detection of ESBL genes, multilocus sequence typing using the Achtman scheme (http://mlst.ucc.ie/mlst/dbs/Ecoli), and virulence-associated gene (VAG) carriage screening by PCR were performed on isolates as described (7).Significantly more bacteremia E. coli isolates than urine E. coli isolates were resistant to ciprofloxacin (25.7% vs. 8.8%; p 2 classes); a significantly higher number of multidrug-resistant bacteremia E. coli isolates than multidrug-resistant urine isolates were found (50.7% vs. 32%; p = 0.01). PCR screening for ESBL carriage showed significantly higher ESBL carriage in bacteremia E. coli isolates than urine isolates for blaSHV (15.7% vs. 5.6%; p = 0.008), blaCTX-M (29.3% vs. 17.6%; p = 0.025), and blaOXA (14.3% vs. 6.4%; p = 0.037). Total ESBL carriage for bacteremia isolates was also significantly higher than for urine isolates (59.3% vs. 29.6%; p<0.001).Multilocus sequence types were determined for all E. coli isolates. A total of 63 STs were found among the urine isolates (Figure, panel A); the highest prevalence was ST73 (n = 16, 12.8%), followed by ST131 (n = 9, 7.2%), ST69 (n = 9, 7.2%), ST95 (n = 6, 4.8%), ST404 (n = 6, 4.8%), ST127 (n = 4, 3.2%), ST141 (n = 4, 3.2%), and ST10 (n = 3, 2.4%). Prevalence patterns of STs among bacteremia E. coli isolates were noticeably different (Figure, panel B). Three main STs were obtained. ST131 dominated (n = 30, 21.43%) and was significantly higher in prevalence than for the urine isolates (p<0.001). ST73 (n = 24, 17.14%) and ST95 (n = 13, 9.29%) were the other 2 primary STs found. The 8 most prevalent STs in the bacteremia isolates represented 59.29% of the total population, whereas the 8 most prevalent STs in the urine isolates represented 45.6% of the total population. This finding is suggestive of selection of a smaller number of dominant STs in bacteremia.FigureMinimum-spanning trees showing carriage of extended-spectrum β-lactamases (ESBL) in Escherichia coli isolates from urine samples (A) and samples from patients with bacteremia (B). Each circle represents 1 sequence type (ST), and the size of the ...ESBL carriage was mapped onto minimum-spanning trees for the 2 isolate groups. ESBL carriage among urine isolates was focused on a small number of STs; 19 (30.16%) of the 63 STs contained ESBL-positive isolates (Figure, panel A). The predominant ST73 group contained 18.75% ESBL-positive isolates; the other predominant STs exhibited ESBL-positive isolates at the following levels: ST131 (44.44%), ST69 (33.33%), ST95 (50%), and ST10 (0%). In contrast, 30 (51.72%) of the 58 STs among bacteremia isolates contained ESBL-positive isolates, significantly higher than for the urine isolates (p = 0.016). At the ST level, predominant STs had higher ESBL carriage in the bacteremia isolates than in the urine isolates: ST131 (50%), ST73 (50%), ST12 (75%), ST10 (100%), ST14 (50%), ST2278 (33.33%). ST95 (46.15%) and ST69 (20%) showed comparable levels. These results suggest that ESBL drug resistance is selecting for dominant ExPEC bacteremia strains.To investigate whether the differences in ST observations between bacteremia and urine isolates could be attributable to differences in virulence genes, VAGs of all isolates were screened by multiplex PCR. VAGs were found equally distributed across the 2 populations, with no statistically significant difference (p = 0.675). Comparison of serum resistance levels between urine and blood isolates also showed no phenotypic differences.In conclusion, we found high levels of ESBL carriage and multidrug resistance in ExPEC isolates that cause bacteremia. A comparison with urine isolates provided evidence that ESBL-mediated drug resistance appears to be the selective pressure in the emergence of dominant STs in bacteremia. Future research should focus on identifying if prolonged antimicrobial drug treatment in bacteremia patients is leading to this selection." @default.
• W2078010237 created "2016-06-24" @default.
• W2078010237 creator A5065207220 @default.
• W2078010237 creator A5067561627 @default.
• W2078010237 creator A5076517379 @default.
• W2078010237 creator A5080540789 @default.
• W2078010237 date "2013-10-01" @default.
• W2078010237 modified "2023-09-28" @default.
• W2078010237 title "Multidrug-Resistant<i>Escherichia coli</i>Bacteremia" @default.
• W2078010237 cites W1797126985 @default.
• W2078010237 cites W1966541844 @default.
• W2078010237 cites W2022178880 @default.
• W2078010237 cites W2094448747 @default.
• W2078010237 cites W2125988863 @default.
• W2078010237 cites W2138078648 @default.
• W2078010237 cites W2144148663 @default.
• W2078010237 doi "https://doi.org/10.3201/eid1910.130309" @default.
• W2078010237 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3810742" @default.
• W2078010237 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24050656" @default.
• W2078010237 hasPublicationYear "2013" @default.
• W2078010237 type Work @default.
• W2078010237 sameAs 2078010237 @default.
• W2078010237 citedByCount "57" @default.
• W2078010237 countsByYear W20780102372014 @default.
• W2078010237 countsByYear W20780102372015 @default.
• W2078010237 countsByYear W20780102372016 @default.
• W2078010237 countsByYear W20780102372017 @default.
• W2078010237 countsByYear W20780102372018 @default.
• W2078010237 countsByYear W20780102372019 @default.
• W2078010237 countsByYear W20780102372020 @default.
• W2078010237 countsByYear W20780102372021 @default.
• W2078010237 countsByYear W20780102372022 @default.
• W2078010237 countsByYear W20780102372023 @default.
• W2078010237 crossrefType "journal-article" @default.
• W2078010237 hasAuthorship W2078010237A5065207220 @default.
• W2078010237 hasAuthorship W2078010237A5067561627 @default.
• W2078010237 hasAuthorship W2078010237A5076517379 @default.
• W2078010237 hasAuthorship W2078010237A5080540789 @default.
• W2078010237 hasBestOaLocation W20780102371 @default.
• W2078010237 hasConcept C104317684 @default.
• W2078010237 hasConcept C114851261 @default.
• W2078010237 hasConcept C133936738 @default.
• W2078010237 hasConcept C159047783 @default.
• W2078010237 hasConcept C2779443120 @default.
• W2078010237 hasConcept C501593827 @default.
• W2078010237 hasConcept C54355233 @default.
• W2078010237 hasConcept C547475151 @default.
• W2078010237 hasConcept C71924100 @default.
• W2078010237 hasConcept C86803240 @default.
• W2078010237 hasConcept C89423630 @default.
• W2078010237 hasConceptScore W2078010237C104317684 @default.
• W2078010237 hasConceptScore W2078010237C114851261 @default.
• W2078010237 hasConceptScore W2078010237C133936738 @default.
• W2078010237 hasConceptScore W2078010237C159047783 @default.
• W2078010237 hasConceptScore W2078010237C2779443120 @default.
• W2078010237 hasConceptScore W2078010237C501593827 @default.
• W2078010237 hasConceptScore W2078010237C54355233 @default.
• W2078010237 hasConceptScore W2078010237C547475151 @default.
• W2078010237 hasConceptScore W2078010237C71924100 @default.
• W2078010237 hasConceptScore W2078010237C86803240 @default.
• W2078010237 hasConceptScore W2078010237C89423630 @default.
• W2078010237 hasIssue "10" @default.
• W2078010237 hasLocation W20780102371 @default.
• W2078010237 hasLocation W20780102372 @default.
• W2078010237 hasLocation W20780102373 @default.
• W2078010237 hasLocation W20780102374 @default.
• W2078010237 hasLocation W20780102375 @default.
• W2078010237 hasOpenAccess W2078010237 @default.
• W2078010237 hasPrimaryLocation W20780102371 @default.
• W2078010237 hasRelatedWork W2013551692 @default.
• W2078010237 hasRelatedWork W2015524974 @default.
• W2078010237 hasRelatedWork W2080910126 @default.
• W2078010237 hasRelatedWork W2158354456 @default.
• W2078010237 hasRelatedWork W2163881342 @default.
• W2078010237 hasRelatedWork W2342146826 @default.
• W2078010237 hasRelatedWork W2359817556 @default.
• W2078010237 hasRelatedWork W2414680297 @default.
• W2078010237 hasRelatedWork W3145958566 @default.
• W2078010237 hasRelatedWork W4200377187 @default.
• W2078010237 hasVolume "19" @default.
• W2078010237 isParatext "false" @default.
• W2078010237 isRetracted "false" @default.
• W2078010237 magId "2078010237" @default.
• W2078010237 workType "article" @default.