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- W2078019352 abstract "β-adrenergic (βAR) receptors belong to the class of G protein-coupled receptors (GPCRs) and generally administrate the physiological response of adrenaline and noradrenaline. They can be classified into three receptor subtypes (β1, β2, β3) which are major drug targets for some of the most commonly prescribed drugs in the history of medicine. The various βAR subtypes also interact differentially with a huge diversity of cytoplasmic (G-proteins, arrestin, adenylate cyclase,⋯) and transmembrane proteins (e.g. receptor homo-/hetero-multimerization, ion channels,⋯) leading to distinctive receptor signaling pathways. Studying such interaction processes of plasma-membrane localized receptor proteins is key for a better understanding of cellular processes. In the past few years, many studies indicated that βAR dimerization can occur between two identical receptors (homodimerization), between two different receptor subtypes of the same family or between receptors of different families (heterodimerization). The physiological role of such di-/multimerizations remains a matter of debate. Here we describe an assay combining TIR (total internal reflection) microscopy and micro-patterned surfaces, which can be used for the detection of protein-protein interactions in and near the cell membrane in-vivo. We studied the homo- and heteromultimerization of β1- and β2-adrenergic receptors. Furthermore we investigated the interaction of βARs with different cytoplasmic signaling proteins such as β-arrestin, G-protein and adenylate cyclase. We unequivocally show that β1ARs as well as β2ARs form homo- and hetero multimers in living CHO-K1 cells. We could also proof the interaction of βARs with β-arrestin, G-protein and adenylate cyclase. In addition we conducted FRAP (fluorescence after photobleaching) experiments for further characterization of these interaction processes. In general, our system is of great interest for a fast and straightforward analysis of membrane-receptor protein interactions." @default.
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- W2078019352 date "2013-01-01" @default.
- W2078019352 modified "2023-09-30" @default.
- W2078019352 title "Protein-Protein Interactions of the β-adrenergic Receptor and Kinetic Analysis of Intracellular Binding Partners via µ-Patterned Surfaces" @default.
- W2078019352 doi "https://doi.org/10.1016/j.bpj.2012.11.185" @default.
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