Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078039462> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2078039462 endingPage "255" @default.
- W2078039462 startingPage "249" @default.
- W2078039462 abstract "Chemiluminescence energy transfer between aminobutylethylisoluminol (ABEI)-biotin and fluorescein-avidin was investigated in order to establish a homogeneous assay for serum biotin in the physiological range. ABEI chemiluminescence was measured at pH 7.4 using microperoxidase-hydrogen peroxide and the chemiluminescence at two wavelengths (460 and 525 nm) measured simultaneously to quantify chemiluminescence energy transfer. ABEI-biotin was synthesized by a mixed anhydride reaction and purified by TLC and HPLC. Binding of ABEI-biotin to fluorescein-avidin resulted in a quenching of the chemiluminescence. Chemiluminescence energy transfer was demonstrated by a 2.5-fold decrease in the ratio of blue (460 nm) to green (525 nm) light emission compared with unbound ABEI-biotin. This energy transfer was used to establish an assay for biotin in the range 1 to 10 nm by relating the concentration of biotin to the ratio of chemiluminescence monitored at 460 and 525 nm simultaneously. The assay was capable of detecting biotin in reference sera and in patients with malabsorption syndromes and chronic alcoholism. The reference range in normal subjects was 1.2 to 4.3 nmol/liter mean ± SD = 2.41 ± 0.91 nmol/liter (n = 20). The quenching of the chemiluminescence of ABEI-biotin when bound to fluorescein-avidin appeared to be the result of a direct interaction between the excited state product of ABEI and fluorescein." @default.
- W2078039462 created "2016-06-24" @default.
- W2078039462 creator A5017139861 @default.
- W2078039462 creator A5038301546 @default.
- W2078039462 date "1986-06-01" @default.
- W2078039462 modified "2023-10-17" @default.
- W2078039462 title "A homogeneous assay for biotin based on chemiluminescence energy transfer" @default.
- W2078039462 cites W1547499122 @default.
- W2078039462 cites W1871752909 @default.
- W2078039462 cites W1950199723 @default.
- W2078039462 cites W1966214454 @default.
- W2078039462 cites W1991516434 @default.
- W2078039462 cites W2005867295 @default.
- W2078039462 cites W2014819128 @default.
- W2078039462 cites W2021117737 @default.
- W2078039462 cites W2069369351 @default.
- W2078039462 cites W2071639838 @default.
- W2078039462 cites W2078702793 @default.
- W2078039462 cites W2094517427 @default.
- W2078039462 cites W2112477361 @default.
- W2078039462 cites W2116954420 @default.
- W2078039462 cites W2131970139 @default.
- W2078039462 cites W2169138268 @default.
- W2078039462 cites W2330555886 @default.
- W2078039462 cites W4211027922 @default.
- W2078039462 doi "https://doi.org/10.1016/0003-2697(86)90433-1" @default.
- W2078039462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3728977" @default.
- W2078039462 hasPublicationYear "1986" @default.
- W2078039462 type Work @default.
- W2078039462 sameAs 2078039462 @default.
- W2078039462 citedByCount "25" @default.
- W2078039462 countsByYear W20780394622013 @default.
- W2078039462 countsByYear W20780394622015 @default.
- W2078039462 countsByYear W20780394622022 @default.
- W2078039462 crossrefType "journal-article" @default.
- W2078039462 hasAuthorship W2078039462A5017139861 @default.
- W2078039462 hasAuthorship W2078039462A5038301546 @default.
- W2078039462 hasConcept C119128265 @default.
- W2078039462 hasConcept C121332964 @default.
- W2078039462 hasConcept C121745418 @default.
- W2078039462 hasConcept C145802702 @default.
- W2078039462 hasConcept C176406525 @default.
- W2078039462 hasConcept C185592680 @default.
- W2078039462 hasConcept C2778015030 @default.
- W2078039462 hasConcept C2778204606 @default.
- W2078039462 hasConcept C43617362 @default.
- W2078039462 hasConcept C533411734 @default.
- W2078039462 hasConcept C55493867 @default.
- W2078039462 hasConcept C62520636 @default.
- W2078039462 hasConcept C75473681 @default.
- W2078039462 hasConcept C91881484 @default.
- W2078039462 hasConceptScore W2078039462C119128265 @default.
- W2078039462 hasConceptScore W2078039462C121332964 @default.
- W2078039462 hasConceptScore W2078039462C121745418 @default.
- W2078039462 hasConceptScore W2078039462C145802702 @default.
- W2078039462 hasConceptScore W2078039462C176406525 @default.
- W2078039462 hasConceptScore W2078039462C185592680 @default.
- W2078039462 hasConceptScore W2078039462C2778015030 @default.
- W2078039462 hasConceptScore W2078039462C2778204606 @default.
- W2078039462 hasConceptScore W2078039462C43617362 @default.
- W2078039462 hasConceptScore W2078039462C533411734 @default.
- W2078039462 hasConceptScore W2078039462C55493867 @default.
- W2078039462 hasConceptScore W2078039462C62520636 @default.
- W2078039462 hasConceptScore W2078039462C75473681 @default.
- W2078039462 hasConceptScore W2078039462C91881484 @default.
- W2078039462 hasIssue "2" @default.
- W2078039462 hasLocation W20780394621 @default.
- W2078039462 hasLocation W20780394622 @default.
- W2078039462 hasOpenAccess W2078039462 @default.
- W2078039462 hasPrimaryLocation W20780394621 @default.
- W2078039462 hasRelatedWork W1491713528 @default.
- W2078039462 hasRelatedWork W2000274299 @default.
- W2078039462 hasRelatedWork W2009843049 @default.
- W2078039462 hasRelatedWork W2012436573 @default.
- W2078039462 hasRelatedWork W2018430038 @default.
- W2078039462 hasRelatedWork W2052203724 @default.
- W2078039462 hasRelatedWork W2081832073 @default.
- W2078039462 hasRelatedWork W2333965755 @default.
- W2078039462 hasRelatedWork W2367619093 @default.
- W2078039462 hasRelatedWork W2417117956 @default.
- W2078039462 hasVolume "155" @default.
- W2078039462 isParatext "false" @default.
- W2078039462 isRetracted "false" @default.
- W2078039462 magId "2078039462" @default.
- W2078039462 workType "article" @default.