FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078061120> ?p ?o ?g. }

• W2078061120 abstract "A cultivation strategy for the production of two truncated thermostable recombinant xylanases (Xyn1deltaN and Xyn1deltaNC) was developed. Fed-batch cultivations of Escherichia coli strain BL21(DE3) with a controlled exponential glucose feed led to high specific production of the recombinant proteins. Addition of complex nutrients (e.g. Tryptone Soya Broth (TSB)) to the media were shown to increase both the specific growth rate during the production phase and the production per cell. The final cell-mass concentration depended on the time of induction in relation to both the feed-start and the expected time at which the cultivation had to be terminated due to oxygen transfer limitations or cell lysis. The gene used for the genetic constructions (encoding Xyn1deltaN and Xyn1deltaNC) was originally isolated from Rhodothermus marinus. Recombinant protein expression was controlled by the T7 lac-promoter and induced in the fed-batch phase at low glucose concentrations by the single addition of either lactose or isopropyl-thio-beta-d-galactoside (IPTG). In lactose-induced cells, the production of recombinant xylanase was delayed for approximately 30 min in comparison with those induced with IPTG, but the specific product levels were comparable at 3 h after induction. At this time, approximately 35% of the intracellular protein content was constituted by recombinant xylanase. Under the cultivation conditions used, production of the shorter deletion derivative (Xyn1deltaNC) led to nonspecific leakage and cell lysis, starting 1.5 or 2 h after induction with IPTG or lactose, respectively. At 3 h after induction, 50% of the produced protein (Xyn1deltaNC) was found in the culture medium. This was not the case for the longer protein (Xyn1deltaN), where only 10% of the xylanase leaked into the medium." @default.
• W2078061120 created "2016-06-24" @default.
• W2078061120 creator A5033042329 @default.
• W2078061120 creator A5035096291 @default.
• W2078061120 creator A5055664768 @default.
• W2078061120 date "1999-01-01" @default.
• W2078061120 modified "2023-09-27" @default.
• W2078061120 title "Efficient production of truncated thermostable xylanases from Rhodothermus marinus in Escherichia coli fed-batch cultures" @default.
• W2078061120 cites W1482938752 @default.
• W2078061120 cites W1775749144 @default.
• W2078061120 cites W1826496811 @default.
• W2078061120 cites W1873199501 @default.
• W2078061120 cites W1963649379 @default.
• W2078061120 cites W1963889064 @default.
• W2078061120 cites W1974809350 @default.
• W2078061120 cites W1975993749 @default.
• W2078061120 cites W1976127558 @default.
• W2078061120 cites W1981323533 @default.
• W2078061120 cites W1981696939 @default.
• W2078061120 cites W2012823593 @default.
• W2078061120 cites W2033134248 @default.
• W2078061120 cites W2033666909 @default.
• W2078061120 cites W2033766977 @default.
• W2078061120 cites W2047462384 @default.
• W2078061120 cites W2056617251 @default.
• W2078061120 cites W2076287670 @default.
• W2078061120 cites W2084261326 @default.
• W2078061120 cites W2090744083 @default.
• W2078061120 cites W2092603929 @default.
• W2078061120 cites W2093969953 @default.
• W2078061120 cites W2099478512 @default.
• W2078061120 cites W2100837269 @default.
• W2078061120 cites W2106203022 @default.
• W2078061120 cites W2112723141 @default.
• W2078061120 cites W2116055160 @default.
• W2078061120 cites W2116654553 @default.
• W2078061120 cites W2167385584 @default.
• W2078061120 cites W2239384431 @default.
• W2078061120 cites W2266502434 @default.
• W2078061120 doi "https://doi.org/10.1016/s1389-1723(99)80121-2" @default.
• W2078061120 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16232525" @default.
• W2078061120 hasPublicationYear "1999" @default.
• W2078061120 type Work @default.
• W2078061120 sameAs 2078061120 @default.
• W2078061120 citedByCount "30" @default.
• W2078061120 countsByYear W20780611202012 @default.
• W2078061120 countsByYear W20780611202015 @default.
• W2078061120 countsByYear W20780611202016 @default.
• W2078061120 countsByYear W20780611202017 @default.
• W2078061120 countsByYear W20780611202018 @default.
• W2078061120 countsByYear W20780611202020 @default.
• W2078061120 countsByYear W20780611202021 @default.
• W2078061120 crossrefType "journal-article" @default.
• W2078061120 hasAuthorship W2078061120A5033042329 @default.
• W2078061120 hasAuthorship W2078061120A5035096291 @default.
• W2078061120 hasAuthorship W2078061120A5055664768 @default.
• W2078061120 hasConcept C100544194 @default.
• W2078061120 hasConcept C104317684 @default.
• W2078061120 hasConcept C167431671 @default.
• W2078061120 hasConcept C170344550 @default.
• W2078061120 hasConcept C181199279 @default.
• W2078061120 hasConcept C2775859485 @default.
• W2078061120 hasConcept C2778724459 @default.
• W2078061120 hasConcept C31903555 @default.
• W2078061120 hasConcept C40767141 @default.
• W2078061120 hasConcept C547475151 @default.
• W2078061120 hasConcept C55493867 @default.
• W2078061120 hasConcept C57409179 @default.
• W2078061120 hasConcept C86803240 @default.
• W2078061120 hasConcept C89423630 @default.
• W2078061120 hasConceptScore W2078061120C100544194 @default.
• W2078061120 hasConceptScore W2078061120C104317684 @default.
• W2078061120 hasConceptScore W2078061120C167431671 @default.
• W2078061120 hasConceptScore W2078061120C170344550 @default.
• W2078061120 hasConceptScore W2078061120C181199279 @default.
• W2078061120 hasConceptScore W2078061120C2775859485 @default.
• W2078061120 hasConceptScore W2078061120C2778724459 @default.
• W2078061120 hasConceptScore W2078061120C31903555 @default.
• W2078061120 hasConceptScore W2078061120C40767141 @default.
• W2078061120 hasConceptScore W2078061120C547475151 @default.
• W2078061120 hasConceptScore W2078061120C55493867 @default.
• W2078061120 hasConceptScore W2078061120C57409179 @default.
• W2078061120 hasConceptScore W2078061120C86803240 @default.
• W2078061120 hasConceptScore W2078061120C89423630 @default.
• W2078061120 hasLocation W20780611201 @default.
• W2078061120 hasLocation W20780611202 @default.
• W2078061120 hasOpenAccess W2078061120 @default.
• W2078061120 hasPrimaryLocation W20780611201 @default.
• W2078061120 hasRelatedWork W1781497060 @default.
• W2078061120 hasRelatedWork W1963903219 @default.
• W2078061120 hasRelatedWork W1976127558 @default.
• W2078061120 hasRelatedWork W1990955571 @default.
• W2078061120 hasRelatedWork W2004865088 @default.
• W2078061120 hasRelatedWork W2006494196 @default.
• W2078061120 hasRelatedWork W2033134248 @default.
• W2078061120 hasRelatedWork W2037592923 @default.
• W2078061120 hasRelatedWork W2047462384 @default.
• W2078061120 hasRelatedWork W2053121582 @default.
• W2078061120 hasRelatedWork W2059020796 @default.
• W2078061120 hasRelatedWork W2061479782 @default.

• next