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- W2078110407 abstract "The application of multivariate curve resolution using the alternating least-squares (ALS) method for the resolution and quantitation of mixtures of two co-eluted compounds is validated for hydrocortisone (main compound) and prednisone (impurity) in liquid chromatography with diode array detection. The recovery of the spectra of the co-eluted compounds and their quantitation are discussed in relation to the initial estimates provided by evolving factor analysis and SIMPLISMA methods. Quantitation errors below 1.5% have been obtained for the main compound for resolution values of 0.4 or above, and impurity concentrations in the range 0.5–20%." @default.
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- W2078110407 title "Validation of alternating least-squares multivariate curve resolution for chromatographic resolution and quantitation" @default.
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