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- W2078116854 abstract "Abstract Escherichia coli 50 S ribosomal subunits lose activity when precipitated by ethanol from fractions obtained in sucrose density gradient sedimentation. This loss of activity is due to a partial extraction of protein L7 L12 caused by the presence of high sucrose concentrations. Partial extraction also occurs in the absence of added salt or sucrose at low magnesium concentrations. Fully active 50 S subunits can be obtained if the sucrose is diluted to a concentration of 10% or less and the magnesium concentration raised to 20 m m before the addition of ethanol." @default.
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- W2078116854 date "1980-07-01" @default.
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- W2078116854 title "Conditions for ethanol precipitation of active 50 S ribosomal subunits from Escherichia coli" @default.
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- W2078116854 doi "https://doi.org/10.1016/0003-2697(80)90137-2" @default.
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