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- W2078120210 abstract "Cell-cycle kinetics of precursors to antibody-forming cells was studied in mouse spleen cell cultures immunized with sheep red cells. Hydroxyurea (0.5 mM) added on the day of culture did not interfere with the response if removed within 24 hr. When it was added on Day 4 of culture, during log growth, 76% of the antibody-forming cells were killed with treatment lasting 8 hr or less. Treatment of 12 hr or more resulted in death for 98% of the cells. The shape of the survival curve showed the proportion of cells killed in S (.74), the duration of S (6–7.5 hr), and generation time 8.5–10 hr. This technique is particularly useful for the study of a very small population of differentiating cells at a time prior to the expression of the unique character by which they are detected." @default.
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- W2078120210 date "1971-04-01" @default.
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- W2078120210 title "Hydroxyurea and cell-cycle kinetics of cultured antibody-forming cells" @default.
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- W2078120210 doi "https://doi.org/10.1016/0008-8749(71)90035-9" @default.
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