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- W2078133694 abstract "The potential of semiconducting nanocrystals or so-called quantum dots (QDs) for lifetime multiplexing has not been investigated yet, despite the increasing use of QDs in (bio)analytical detection, biosensing, and fluorescence imaging and the obvious need for simple and cost-effective tools and strategies for the simultaneous detection of multiple analytes or events. This is most likely related to their multiexponential decay behavior as for multiplex chromophores, typically monoexponential decay kinetics are requested. The fluorescence decay kinetics of various mixtures of a long-lived, multiexponentially decaying CdSe QD and a short-lived organic dye were analyzed, and a model was developed for the quantification of these labels from the measured complex decay kinetics as a first proof-of-concept for the huge potential of these labels for lifetime multiplexing. In a second step, we evaluated the potential of mixtures of two types of QDs, varying in constituent material to realize distinguishable, yet multiexponential decay kinetics and similar absorption and emission spectra. Strategies for lifetime multiplexing with nanocrystalline labels were derived on the basis of these measurements." @default.
- W2078133694 created "2016-06-24" @default.
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- W2078133694 date "2009-08-25" @default.
- W2078133694 modified "2023-09-27" @default.
- W2078133694 title "Fluorescence Lifetime Multiplexing with Nanocrystals and Organic Labels" @default.
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- W2078133694 doi "https://doi.org/10.1021/ac900934a" @default.
- W2078133694 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19705851" @default.
- W2078133694 hasPublicationYear "2009" @default.
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