Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078137379> ?p ?o ?g. }
- W2078137379 endingPage "50" @default.
- W2078137379 startingPage "37" @default.
- W2078137379 abstract "Biological active cDNA clones of cucumber mosaic virus (CMV) RNAs 1 and 2 were modified by addition of sequences that encode hexahistidine (His-tag) at the amino- (N-) or carboxy- (C-) terminus of the 1a and 2a proteins. These proteins are essential components of the viral RNA-dependent RNA polymerase (RdRp). In all but one case, addition of the His-tag did not significantly affect the yields of the corresponding viruses and the His-tag-encoding sequences were maintained after mechanical passages. No differences were observed among the in vitro activities of the modified vs. wild-type viral RdRps. Subcellular fractionation showed that 2a protein was found both membrane-associated and in the 30,000 x g soluble fraction. Both termini of the native His-tag 2a protein could bind to a resin containing nickel-nitrilotriacetic acid (Ni(2+)-NTA). Detergent-treated RdRp containing C-terminal His-tagged 1a and 2a proteins was chromatographed on Ni(2+)-NTA resin. The activity of the eluted RdRp was template- dependent, in contrast to pre-chromatography fractions. However, only a small proportion of the viral RdRp as well as numerous host proteins bound to and eluted from the resin under non-denaturing conditions." @default.
- W2078137379 created "2016-06-24" @default.
- W2078137379 creator A5011570757 @default.
- W2078137379 creator A5030690898 @default.
- W2078137379 creator A5068872525 @default.
- W2078137379 date "2000-01-01" @default.
- W2078137379 modified "2023-09-26" @default.
- W2078137379 title "Characterisation of genetically modified cucumber mosaic virus expressing histidine-tagged 1a and 2a proteins" @default.
- W2078137379 cites W1482767536 @default.
- W2078137379 cites W1596323100 @default.
- W2078137379 cites W1789760598 @default.
- W2078137379 cites W1792302550 @default.
- W2078137379 cites W1979214049 @default.
- W2078137379 cites W1988610156 @default.
- W2078137379 cites W1996180485 @default.
- W2078137379 cites W2000568649 @default.
- W2078137379 cites W2002742341 @default.
- W2078137379 cites W2004378478 @default.
- W2078137379 cites W2008443324 @default.
- W2078137379 cites W2009188702 @default.
- W2078137379 cites W2010588708 @default.
- W2078137379 cites W2018566575 @default.
- W2078137379 cites W2027271983 @default.
- W2078137379 cites W2029104347 @default.
- W2078137379 cites W2029309604 @default.
- W2078137379 cites W2029449095 @default.
- W2078137379 cites W2033303857 @default.
- W2078137379 cites W2038793880 @default.
- W2078137379 cites W2041538257 @default.
- W2078137379 cites W2044524906 @default.
- W2078137379 cites W2051519304 @default.
- W2078137379 cites W2055697147 @default.
- W2078137379 cites W2058191470 @default.
- W2078137379 cites W2069788839 @default.
- W2078137379 cites W2070919514 @default.
- W2078137379 cites W2078126117 @default.
- W2078137379 cites W2094670154 @default.
- W2078137379 cites W2101108802 @default.
- W2078137379 cites W2114252822 @default.
- W2078137379 cites W2118612144 @default.
- W2078137379 cites W2137294277 @default.
- W2078137379 cites W2143762209 @default.
- W2078137379 cites W2146504848 @default.
- W2078137379 cites W2146792916 @default.
- W2078137379 cites W2155982042 @default.
- W2078137379 cites W2164492221 @default.
- W2078137379 cites W2169192961 @default.
- W2078137379 cites W2332649652 @default.
- W2078137379 cites W2888511473 @default.
- W2078137379 cites W2963673177 @default.
- W2078137379 cites W299955650 @default.
- W2078137379 doi "https://doi.org/10.1007/s007050050003" @default.
- W2078137379 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10664404" @default.
- W2078137379 hasPublicationYear "2000" @default.
- W2078137379 type Work @default.
- W2078137379 sameAs 2078137379 @default.
- W2078137379 citedByCount "23" @default.
- W2078137379 countsByYear W20781373792012 @default.
- W2078137379 countsByYear W20781373792017 @default.
- W2078137379 countsByYear W20781373792018 @default.
- W2078137379 countsByYear W20781373792021 @default.
- W2078137379 countsByYear W20781373792022 @default.
- W2078137379 countsByYear W20781373792023 @default.
- W2078137379 crossrefType "journal-article" @default.
- W2078137379 hasAuthorship W2078137379A5011570757 @default.
- W2078137379 hasAuthorship W2078137379A5030690898 @default.
- W2078137379 hasAuthorship W2078137379A5068872525 @default.
- W2078137379 hasBestOaLocation W20781373791 @default.
- W2078137379 hasConcept C104317684 @default.
- W2078137379 hasConcept C109110057 @default.
- W2078137379 hasConcept C153911025 @default.
- W2078137379 hasConcept C159047783 @default.
- W2078137379 hasConcept C178790620 @default.
- W2078137379 hasConcept C185592680 @default.
- W2078137379 hasConcept C197404232 @default.
- W2078137379 hasConcept C2522874641 @default.
- W2078137379 hasConcept C2776449523 @default.
- W2078137379 hasConcept C2776513250 @default.
- W2078137379 hasConcept C2778306172 @default.
- W2078137379 hasConcept C2778460671 @default.
- W2078137379 hasConcept C2779842363 @default.
- W2078137379 hasConcept C515207424 @default.
- W2078137379 hasConcept C55493867 @default.
- W2078137379 hasConcept C67705224 @default.
- W2078137379 hasConcept C86803240 @default.
- W2078137379 hasConceptScore W2078137379C104317684 @default.
- W2078137379 hasConceptScore W2078137379C109110057 @default.
- W2078137379 hasConceptScore W2078137379C153911025 @default.
- W2078137379 hasConceptScore W2078137379C159047783 @default.
- W2078137379 hasConceptScore W2078137379C178790620 @default.
- W2078137379 hasConceptScore W2078137379C185592680 @default.
- W2078137379 hasConceptScore W2078137379C197404232 @default.
- W2078137379 hasConceptScore W2078137379C2522874641 @default.
- W2078137379 hasConceptScore W2078137379C2776449523 @default.
- W2078137379 hasConceptScore W2078137379C2776513250 @default.
- W2078137379 hasConceptScore W2078137379C2778306172 @default.
- W2078137379 hasConceptScore W2078137379C2778460671 @default.
- W2078137379 hasConceptScore W2078137379C2779842363 @default.