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- W2078147272 abstract "The cellular DNA's of Dictyostelium discoideum have been characterized by their behavior in CsCl buoyant density-gradient centrifugation and by their renaturation kinetics. Whole-cell DNA exhibited three bands on CsCl density-gradient centrifugation. Two of these, p = 1.676 g/cm3 (main band) and 1.687 (satellite) are nuclear. The third, of p = 1.682 is mitochondrial and represents approximately 28% of whole-cell DNA. Appropriate hybridization experiments with cytoplasmic rRNA show that rRNA cistrons constitute 2.2% of nuclear DNA. This portion of the nuclear DNA possesses a ρ of approximately 1.682 and bands in the mitochondrial DNA region. Non-reiterated sequences make up approximately 70% of the Dictyostelium genome (criterion of Tm − 23 deg. C) and are characterized by a C0t12 (pure) of 70. Reiterated sequences make up 30% of the genome and are characterized by a C0t12 (pure) of 0.28. The complexity of the nuclear DNA of Dictyostelium is but 11 times that of Escherichia coli (approximately 30 ×109 daltons). The mitochondrial DNA of Dictyostelium exhibits a C0t12 of 0.15 and a calculated complexity of 35 to 40 × 106 daltons." @default.
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- W2078147272 date "1972-05-01" @default.
- W2078147272 modified "2023-10-16" @default.
- W2078147272 title "Characterization of the genome of the cellular slime mold Dictyostelium discoideum" @default.
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- W2078147272 doi "https://doi.org/10.1016/0022-2836(72)90419-6" @default.
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