FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078159744> ?p ?o ?g. }

• W2078159744 endingPage "1318" @default.
• W2078159744 startingPage "1318" @default.
• W2078159744 abstract "In this contribution, we demonstrate that polystyrene (PS) nanospheres can serve as an effective sensing platform for fluorescence-enhanced DNA detection. This kind of assay can be completed by the following two steps: (1) PS quenches the fluorescence of dye-labeled single-stranded DNA (ssDNA) probes very effectively when they are brought into close proximity as a result of the adsorption of ssDNA on PS. The adsorption is ascribed to the strong π–π stacking between unpaired DNA bases and PS. (2) Upon presence of target ssDNA, the specific hybridization of the probe with its target produces a double-stranded DNA (dsDNA). The duplex detaches from PS due to its rigid conformation, the absence of unpaired DNA bases, and electrostatic repulsion between negatively charged dsDNA backbone and PS, leading to recovery of dye fluorescence. This assay system exhibits high selectivity and sensitivity with a detection limit as low as 5 nM. It suggests that this sensing platform can differentiate between perfectly complementary and mismatched targets. The fluorescence enhancement in response to single-base mismatched target T2, two-base mismatched target T3, and three-base mismatched target T4 is about 71%, 63%, and 58% of that from complementary target T1, respectively. The suggested method can also discriminate complementary and single-base mismatched sequences embedded in rather larger strands with short oligonucleotide probes. The fluorescence enhancement in response to single-base mismatched sequence embedded in large strand is 83% of that from the one embedded with complementary sequence. In further experiments, it is demonstrated that the present system can be used for multiple DNA detection. Finally, efforts are made toward its application in human blood serum system to evaluate the ability to withstand the interference arising from real sample." @default.
• W2078159744 created "2016-06-24" @default.
• W2078159744 creator A5022154319 @default.
• W2078159744 creator A5056556778 @default.
• W2078159744 creator A5056725318 @default.
• W2078159744 creator A5059912872 @default.
• W2078159744 creator A5062916226 @default.
• W2078159744 creator A5073216396 @default.
• W2078159744 date "2011-01-01" @default.
• W2078159744 modified "2023-10-18" @default.
• W2078159744 title "Detection of single-stranded nucleic acids by hybridization of probe oligonucleotides on polystyrene nanospheres and subsequent release and recovery of fluorescence" @default.
• W2078159744 cites W1631110814 @default.
• W2078159744 cites W1963853131 @default.
• W2078159744 cites W1968832588 @default.
• W2078159744 cites W1970279897 @default.
• W2078159744 cites W1975342005 @default.
• W2078159744 cites W1975418456 @default.
• W2078159744 cites W1981002967 @default.
• W2078159744 cites W1981211116 @default.
• W2078159744 cites W1981391530 @default.
• W2078159744 cites W1982636587 @default.
• W2078159744 cites W1983463436 @default.
• W2078159744 cites W1984649408 @default.
• W2078159744 cites W1985941691 @default.
• W2078159744 cites W1991624307 @default.
• W2078159744 cites W1998313929 @default.
• W2078159744 cites W2001280311 @default.
• W2078159744 cites W2003374280 @default.
• W2078159744 cites W2004439252 @default.
• W2078159744 cites W2011298634 @default.
• W2078159744 cites W2012038939 @default.
• W2078159744 cites W2025069292 @default.
• W2078159744 cites W2040057549 @default.
• W2078159744 cites W2042286794 @default.
• W2078159744 cites W2046334965 @default.
• W2078159744 cites W2048755369 @default.
• W2078159744 cites W2052156135 @default.
• W2078159744 cites W2052480089 @default.
• W2078159744 cites W2055873038 @default.
• W2078159744 cites W2061755273 @default.
• W2078159744 cites W2064062630 @default.
• W2078159744 cites W2071659422 @default.
• W2078159744 cites W2074711360 @default.
• W2078159744 cites W2078820160 @default.
• W2078159744 cites W2084731092 @default.
• W2078159744 cites W2085879142 @default.
• W2078159744 cites W2095273793 @default.
• W2078159744 cites W2097884197 @default.
• W2078159744 cites W2111693505 @default.
• W2078159744 cites W2114694243 @default.
• W2078159744 cites W2115568341 @default.
• W2078159744 cites W2129304273 @default.
• W2078159744 cites W2148801431 @default.
• W2078159744 cites W2171260598 @default.
• W2078159744 doi "https://doi.org/10.1039/c1ra00241d" @default.
• W2078159744 hasPublicationYear "2011" @default.
• W2078159744 type Work @default.
• W2078159744 sameAs 2078159744 @default.
• W2078159744 citedByCount "6" @default.
• W2078159744 countsByYear W20781597442012 @default.
• W2078159744 countsByYear W20781597442016 @default.
• W2078159744 countsByYear W20781597442017 @default.
• W2078159744 countsByYear W20781597442018 @default.
• W2078159744 crossrefType "journal-article" @default.
• W2078159744 hasAuthorship W2078159744A5022154319 @default.
• W2078159744 hasAuthorship W2078159744A5056556778 @default.
• W2078159744 hasAuthorship W2078159744A5056725318 @default.
• W2078159744 hasAuthorship W2078159744A5059912872 @default.
• W2078159744 hasAuthorship W2078159744A5062916226 @default.
• W2078159744 hasAuthorship W2078159744A5073216396 @default.
• W2078159744 hasConcept C105795698 @default.
• W2078159744 hasConcept C119128265 @default.
• W2078159744 hasConcept C121332964 @default.
• W2078159744 hasConcept C12554922 @default.
• W2078159744 hasConcept C129312508 @default.
• W2078159744 hasConcept C130197536 @default.
• W2078159744 hasConcept C178790620 @default.
• W2078159744 hasConcept C185581394 @default.
• W2078159744 hasConcept C185592680 @default.
• W2078159744 hasConcept C21951064 @default.
• W2078159744 hasConcept C24107716 @default.
• W2078159744 hasConcept C2777922577 @default.
• W2078159744 hasConcept C32795469 @default.
• W2078159744 hasConcept C33347731 @default.
• W2078159744 hasConcept C33923547 @default.
• W2078159744 hasConcept C43617362 @default.
• W2078159744 hasConcept C521977710 @default.
• W2078159744 hasConcept C552990157 @default.
• W2078159744 hasConcept C55493867 @default.
• W2078159744 hasConcept C62520636 @default.
• W2078159744 hasConcept C86803240 @default.
• W2078159744 hasConcept C91881484 @default.
• W2078159744 hasConcept C99611785 @default.
• W2078159744 hasConceptScore W2078159744C105795698 @default.
• W2078159744 hasConceptScore W2078159744C119128265 @default.
• W2078159744 hasConceptScore W2078159744C121332964 @default.
• W2078159744 hasConceptScore W2078159744C12554922 @default.
• W2078159744 hasConceptScore W2078159744C129312508 @default.

• next