FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078162078> ?p ?o ?g. }

• W2078162078 endingPage "175" @default.
• W2078162078 startingPage "169" @default.
• W2078162078 abstract "RNA interference (RNAi) provides a promising therapeutic approach to human diseases. However, data from recent reports demonstrate that short-hairpin RNAs (shRNAs) may cause cellular toxicity, and this warrants further investigation of the safety of using RNAi vectors. Earlier, in comparing hairpin-based RNAi vectors, we noted that shRNAs are highly expressed and yield an abundance of unprocessed precursors, whereas artificial microRNAs (miRNAs) are expressed at lower levels and are processed efficiently. We hypothesized that unprocessed shRNAs arise from the saturation of endogenous RNAi machinery, which poses likely a burden to cells. In this study, we tested that hypothesis by assessing the relative effects of shRNAs and artificial miRNAs on the processing and function of miRNAs. In competition assays, shRNAs disrupted miRNA biogenesis and function, whereas artificial miRNAs avoided this interference even when dosed to silence as effectively as shRNAs. We next compared the safety of these vectors in mouse cerebella, and found that shRNAs cause Purkinje cell neurotoxicity. By contrast, artificial miRNA expression was well tolerated, resulting in effective target gene silencing in Purkinje cells. These findings, together with data from earlier work in mouse striata, suggest that miRNA-based platforms are better suited for therapeutic silencing in the mammalian brain." @default.
• W2078162078 created "2016-06-24" @default.
• W2078162078 creator A5017176346 @default.
• W2078162078 creator A5044981665 @default.
• W2078162078 creator A5088133509 @default.
• W2078162078 date "2009-01-01" @default.
• W2078162078 modified "2023-10-16" @default.
• W2078162078 title "Artificial MicroRNAs as siRNA Shuttles: Improved Safety as Compared to shRNAs In vitro and In vivo" @default.
• W2078162078 cites W1611419331 @default.
• W2078162078 cites W1622884348 @default.
• W2078162078 cites W1647075334 @default.
• W2078162078 cites W1789096095 @default.
• W2078162078 cites W1967633197 @default.
• W2078162078 cites W1971643509 @default.
• W2078162078 cites W1979428788 @default.
• W2078162078 cites W1979837223 @default.
• W2078162078 cites W1980269624 @default.
• W2078162078 cites W1993883459 @default.
• W2078162078 cites W2004817863 @default.
• W2078162078 cites W2014064723 @default.
• W2078162078 cites W2042739691 @default.
• W2078162078 cites W2043828851 @default.
• W2078162078 cites W2045402423 @default.
• W2078162078 cites W2061113358 @default.
• W2078162078 cites W2066173314 @default.
• W2078162078 cites W2071596792 @default.
• W2078162078 cites W2072543946 @default.
• W2078162078 cites W2082213106 @default.
• W2078162078 cites W2087453865 @default.
• W2078162078 cites W2092371719 @default.
• W2078162078 cites W2104979554 @default.
• W2078162078 cites W2106929712 @default.
• W2078162078 cites W2107235611 @default.
• W2078162078 cites W2116660518 @default.
• W2078162078 cites W2118778532 @default.
• W2078162078 cites W2125881252 @default.
• W2078162078 cites W2138708625 @default.
• W2078162078 cites W2142176232 @default.
• W2078162078 cites W2149291473 @default.
• W2078162078 cites W2157741650 @default.
• W2078162078 cites W2160063406 @default.
• W2078162078 cites W2174590326 @default.
• W2078162078 doi "https://doi.org/10.1038/mt.2008.231" @default.
• W2078162078 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2834985" @default.
• W2078162078 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19002161" @default.
• W2078162078 hasPublicationYear "2009" @default.
• W2078162078 type Work @default.
• W2078162078 sameAs 2078162078 @default.
• W2078162078 citedByCount "316" @default.
• W2078162078 countsByYear W20781620782012 @default.
• W2078162078 countsByYear W20781620782013 @default.
• W2078162078 countsByYear W20781620782014 @default.
• W2078162078 countsByYear W20781620782015 @default.
• W2078162078 countsByYear W20781620782016 @default.
• W2078162078 countsByYear W20781620782017 @default.
• W2078162078 countsByYear W20781620782018 @default.
• W2078162078 countsByYear W20781620782019 @default.
• W2078162078 countsByYear W20781620782020 @default.
• W2078162078 countsByYear W20781620782021 @default.
• W2078162078 countsByYear W20781620782022 @default.
• W2078162078 countsByYear W20781620782023 @default.
• W2078162078 crossrefType "journal-article" @default.
• W2078162078 hasAuthorship W2078162078A5017176346 @default.
• W2078162078 hasAuthorship W2078162078A5044981665 @default.
• W2078162078 hasAuthorship W2078162078A5088133509 @default.
• W2078162078 hasBestOaLocation W20781620781 @default.
• W2078162078 hasConcept C104317684 @default.
• W2078162078 hasConcept C119056186 @default.
• W2078162078 hasConcept C145059251 @default.
• W2078162078 hasConcept C166703698 @default.
• W2078162078 hasConcept C190232843 @default.
• W2078162078 hasConcept C22615655 @default.
• W2078162078 hasConcept C2776802755 @default.
• W2078162078 hasConcept C2779867133 @default.
• W2078162078 hasConcept C54355233 @default.
• W2078162078 hasConcept C67705224 @default.
• W2078162078 hasConcept C70721500 @default.
• W2078162078 hasConcept C86803240 @default.
• W2078162078 hasConcept C95444343 @default.
• W2078162078 hasConceptScore W2078162078C104317684 @default.
• W2078162078 hasConceptScore W2078162078C119056186 @default.
• W2078162078 hasConceptScore W2078162078C145059251 @default.
• W2078162078 hasConceptScore W2078162078C166703698 @default.
• W2078162078 hasConceptScore W2078162078C190232843 @default.
• W2078162078 hasConceptScore W2078162078C22615655 @default.
• W2078162078 hasConceptScore W2078162078C2776802755 @default.
• W2078162078 hasConceptScore W2078162078C2779867133 @default.
• W2078162078 hasConceptScore W2078162078C54355233 @default.
• W2078162078 hasConceptScore W2078162078C67705224 @default.
• W2078162078 hasConceptScore W2078162078C70721500 @default.
• W2078162078 hasConceptScore W2078162078C86803240 @default.
• W2078162078 hasConceptScore W2078162078C95444343 @default.
• W2078162078 hasIssue "1" @default.
• W2078162078 hasLocation W20781620781 @default.
• W2078162078 hasLocation W20781620782 @default.
• W2078162078 hasLocation W20781620783 @default.
• W2078162078 hasLocation W20781620784 @default.
• W2078162078 hasOpenAccess W2078162078 @default.

• next