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• W2078166220 abstract "Rats treated with cycloheximide accumulate cholesterol in the adrenal mitochondria. This cholesterol is largely associated with the mitochondrial outer membrane. The addition of homogeneous sterol carrier protein2 (SCP2) to these mitochondria stimulated pregnenolone production to a greater extent than with mitochondria from untreated rats. Adrenal mitochondria from cycloheximide-treated rats were incubated in the presence of aminoglutethimide and cycloheximide to prevent further utilization of cholesterol for pregnenolone synthesis. Outer and inner membrane fractions of these mitochondria incubated with and without 0.75 microM SCP2, were prepared following hypotonic disruption. Incubations of mitochondria in the presence of SCP2 caused a marked shift in cholesterol from outer to inner membrane fractions. The findings provide direct evidence for a role of SCP2 or a similar peptide in modulating transfer of cholesterol to the inner membrane site of cholesterol side chain cleavage." @default.
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• W2078166220 date "1984-07-01" @default.
• W2078166220 modified "2023-09-26" @default.
• W2078166220 title "Sterol carrier protein2 (SCP2)-mediated transfer of cholesterol to mitochondrial inner membranes" @default.
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• W2078166220 doi "https://doi.org/10.1016/s0006-291x(84)80062-5" @default.
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