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- W2078166432 abstract "Contrary to a previous report [J. Viña, R. Hems and H. A. Krebs, Biochem. J.170, 627 (1978)], we have observed that incubation of isolated rat hepatocytes in a medium containing high concentrations of cysteine did not deplete intracellular glutathione (GSH), but instead caused a net increase of GSH. Hepatocyte-dependent accumulation of the mixed disulfide of cysteine and GSH (CYSSG) in incubation media within a 2-hr period was increased several-fold in medium containing cysteine or cystine compared to methionine-containing medium. Hepatocytes (106 cells/ml) placed in medium containing normal levels of cysteine, cystine, or methionine established about a 1:1 ratio of extracellular GSH to GSSG at a concentration of about 6μM each. These results indicate a possible intracellular-extracellular relationship for glutathione and an intracellular response to the extracellular status of glutathione and cyst(e)ine." @default.
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- W2078166432 date "1981-06-01" @default.
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- W2078166432 title "Influence of cysteine upon the glutathione status of isolated rat hepatocytes" @default.
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- W2078166432 doi "https://doi.org/10.1016/0006-2952(81)90302-6" @default.
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