FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078173737> ?p ?o ?g. }

• W2078173737 endingPage "140" @default.
• W2078173737 startingPage "133" @default.
• W2078173737 abstract "GABA transporters accumulate GABA to inactivate or reutilize it. Transporter-mediated GABA release can also occur. Recent findings indicate that GABA transporters can perform additional functions. We investigated how activation of GABA transporters can mediate release of glycine. Nerve endings purified from mouse cerebellum were prelabeled with [3H]glycine in presence of the glycine GlyT1 transporter inhibitor NFPS to label selectively GlyT2-bearing terminals. GABA was added under superfusion conditions and the mechanisms of the GABA-evoked [3H]glycine release were characterized. GABA stimulated [3H]glycine release in a concentration-dependent manner (EC50 = 8.26 μM). The GABA-evoked release was insensitive to GABAA and GABAB receptor antagonists, but it was abolished by GABA transporter inhibitors. About 25% of the evoked release was dependent on external Ca2+entering the nerve terminals through VSCCs sensitive to ω-conotoxins. The external Ca2+-independent release involved mitochondrial Ca2+, as it was prevented by the Na+/Ca2+exchanger inhibitor CGP37157. The GABA uptake-mediated increases in cytosolic Ca2+ did not trigger exocytotic release because the [3H]glycine efflux was insensitive to clostridial toxins. Bafilomycin inhibited the evoked release likely because it reduced vesicular storage of [3H]glycine so that less [3H]glycine can become cytosolic when GABA taken up exchanges with [3H]glycine at the vesicular inhibitory amino acid transporters shared by the two amino acids. The GABA-evoked [3H]glycine efflux could be prevented by niflumic acid or NPPB indicating that the evoked release occurred essentially by permeation through anion channels. In conclusion, GABA uptake into GlyT2-bearing cerebellar nerve endings triggered glycine release which occurred essentially by permeation through Ca2+-dependent anion channels. Glial GABA release mediated by anion channels was proposed to underlie tonic inhibition in the cerebellum; the present results suggest that glycine release by neuronal anion channels also might contribute to tonic inhibition." @default.
• W2078173737 created "2016-06-24" @default.
• W2078173737 creator A5007320925 @default.
• W2078173737 creator A5015779278 @default.
• W2078173737 creator A5040887321 @default.
• W2078173737 date "2012-07-01" @default.
• W2078173737 modified "2023-10-16" @default.
• W2078173737 title "GABA transporters mediate glycine release from cerebellum nerve endings: Roles of Ca2+channels, mitochondrial Na+/Ca2+ exchangers, vesicular GABA/glycine transporters and anion channels" @default.
• W2078173737 cites W1520127837 @default.
• W2078173737 cites W1788084167 @default.
• W2078173737 cites W1840415699 @default.
• W2078173737 cites W1852646540 @default.
• W2078173737 cites W1862870409 @default.
• W2078173737 cites W1938064199 @default.
• W2078173737 cites W1964763649 @default.
• W2078173737 cites W1971264177 @default.
• W2078173737 cites W1972653771 @default.
• W2078173737 cites W1976683130 @default.
• W2078173737 cites W1977265431 @default.
• W2078173737 cites W1981111905 @default.
• W2078173737 cites W1981261198 @default.
• W2078173737 cites W1984633991 @default.
• W2078173737 cites W1992792117 @default.
• W2078173737 cites W1993004704 @default.
• W2078173737 cites W1995684052 @default.
• W2078173737 cites W1996957734 @default.
• W2078173737 cites W1997631508 @default.
• W2078173737 cites W1998388042 @default.
• W2078173737 cites W2002367189 @default.
• W2078173737 cites W2007337191 @default.
• W2078173737 cites W2016321438 @default.
• W2078173737 cites W2019974245 @default.
• W2078173737 cites W2021706455 @default.
• W2078173737 cites W2027175844 @default.
• W2078173737 cites W2028649508 @default.
• W2078173737 cites W2031598294 @default.
• W2078173737 cites W2032770343 @default.
• W2078173737 cites W2039455021 @default.
• W2078173737 cites W2039978771 @default.
• W2078173737 cites W2043436653 @default.
• W2078173737 cites W2045612056 @default.
• W2078173737 cites W2046196975 @default.
• W2078173737 cites W2049879893 @default.
• W2078173737 cites W2053739364 @default.
• W2078173737 cites W2058011414 @default.
• W2078173737 cites W2060224220 @default.
• W2078173737 cites W2060890290 @default.
• W2078173737 cites W2065031748 @default.
• W2078173737 cites W2068675667 @default.
• W2078173737 cites W2072465203 @default.
• W2078173737 cites W2074382485 @default.
• W2078173737 cites W2079610613 @default.
• W2078173737 cites W2081678712 @default.
• W2078173737 cites W2081811399 @default.
• W2078173737 cites W2084474788 @default.
• W2078173737 cites W2086655442 @default.
• W2078173737 cites W2088093088 @default.
• W2078173737 cites W2090489687 @default.
• W2078173737 cites W2094923905 @default.
• W2078173737 cites W2115884335 @default.
• W2078173737 cites W2116876553 @default.
• W2078173737 cites W2117226055 @default.
• W2078173737 cites W2132457456 @default.
• W2078173737 cites W2134779286 @default.
• W2078173737 cites W2138788537 @default.
• W2078173737 cites W2161917727 @default.
• W2078173737 cites W2165917097 @default.
• W2078173737 cites W2167719316 @default.
• W2078173737 cites W2170694961 @default.
• W2078173737 cites W69341822 @default.
• W2078173737 cites W77302046 @default.
• W2078173737 doi "https://doi.org/10.1016/j.neuint.2012.05.005" @default.
• W2078173737 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22579572" @default.
• W2078173737 hasPublicationYear "2012" @default.
• W2078173737 type Work @default.
• W2078173737 sameAs 2078173737 @default.
• W2078173737 citedByCount "12" @default.
• W2078173737 countsByYear W20781737372013 @default.
• W2078173737 countsByYear W20781737372014 @default.
• W2078173737 countsByYear W20781737372015 @default.
• W2078173737 countsByYear W20781737372016 @default.
• W2078173737 countsByYear W20781737372017 @default.
• W2078173737 countsByYear W20781737372021 @default.
• W2078173737 crossrefType "journal-article" @default.
• W2078173737 hasAuthorship W2078173737A5007320925 @default.
• W2078173737 hasAuthorship W2078173737A5015779278 @default.
• W2078173737 hasAuthorship W2078173737A5040887321 @default.
• W2078173737 hasConcept C104317684 @default.
• W2078173737 hasConcept C12554922 @default.
• W2078173737 hasConcept C149011108 @default.
• W2078173737 hasConcept C170493617 @default.
• W2078173737 hasConcept C181199279 @default.
• W2078173737 hasConcept C185592680 @default.
• W2078173737 hasConcept C202751555 @default.
• W2078173737 hasConcept C2776219046 @default.
• W2078173737 hasConcept C2777756961 @default.
• W2078173737 hasConcept C2777771985 @default.
• W2078173737 hasConcept C2778921374 @default.

• next