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• W2078175312 abstract "ResumenEste trabajo demuestra que la estimacion del numero de sitios receptores testiculares (Bmax) a LH/hCG y de su constante de disociacion (Kd) son afectados por la dilucion de la reaccion. Las implicaciones de estos resultados fueron consideradas en relacion a los estudios de un factor inhibidor de la union de LH/hCG a receptores gonadales, obtenido de un extracto acuoso de testiculo de rata. Se demostro que despues de corregir el efecto de dilucion producido por la incubacion y el lavado de la reaccion, 200 μl. del extracto testicular era capaz de inhibir la formacion del complejo de hCG-receptor en un 35% (P < 0,0005). La no consideracion del efecto de dilucion hubiera conducido a la interpretacion, que el factor inhibidor ejercia tambien un efecto significativo sobre la disociacion de dicho complejo (28%, P < 0,001). Estas observaciones fueron inherentes a la reaccion hormona-receptor ya que en la ausencia de hCG, no se detecto ningun efecto por la incubacion o por el lavado sobre la preparacion de receptores. Se enfatiza, por lo tanto, la importancia de considerar los efectos de dilucion de la reaccion que ocurren cuando se usan volumenes de reaccion grandes, o cuando se lava la preparacion de receptores una vez completada la reaccion de union con la hormona.ZusammenfassungWechselwirkungen zwischen testikularen Gonadotropin-Rezeptoren bei der Ratte und menschlichem Choriongonadotropin: Einflus der experimentellen VerdunnungDie Schatzung der Anzahl testikularer Rezeptoren fur die Bindung von LH/HCG bei der Ratte (Bmax) und die Dissoziationskonstante (Kd) sind abhangig vom Reaktionsvolumen des Testansatzes. Ursache fur dieses Phanomen ist ein Faktor, der aus dem Uberstand (105,000 g) eines Ratten-Hodenextraktes gewonnen werden kann und der bei der Bildung des Hormon-Rezeptor-Komplexes zu interferieren vermag. Werden keine Korrekturen bezuglich der Verdunnung gemacht, so resultiert durch diesen Faktor auch ein signifikanter Einflus auf die Dissoziation des Komplexes (p < 0,001). Aufgrund dieser Beobachtungen ergibt sich die Notwendigkeit, bei Gonadotropin-Bindungs-Assays durch adaquate Kontrollen den Verdunnungseffekt zu korrigieren.SummaryInteraction of rat testicular receptors with human chorionic gonadotrophin: Experimental implications of the effects of dilutionThe effects of reaction volume and washing upon the binding of human chorionic gonadotrophin (hCG) to rat testicular receptors were studied. It was demonstrated that the number of detectable hormone-binding sites (Bmax) and the apparent equilibrium dissociation constant (Kd) were highly dependent upon the reaction volume in which the binding assays were carried out. Thus, the results suggest that caution be exercised in the application of the rapid equilibrium model as a true representation of the actual binding interaction between gonadotrophins and particulate receptors. These findings prompted us to re-examine the studies with respect to a factor obtained from a 105,000 g. supernatant of rat testis which inhibits hCG binding to gonadal receptors. It was revealed that after correction for dilution of the binding reaction, the binding inhibitor prevented hCG binding principally, if not entirely, by interfering with the formation of the hormone-receptor complex. However, if no such corrections were made, then the binding inhibitor also appeared to exert a significant effect upon dissociation of the complex (28 p%; P < 0.001). Our results, therefore, emphasize the need to introduce the appropriate controls to correct for the dilution effects occurring during gonadotrophin-binding assay studies.ResumeInteractions des recepteurs testiculaires du rat avec la gonadotropine chorionique humaine: Implications experimentales des effets de dilutionl'evaluation du nombre des recepteurs testiculaires pour la liaison de LH/HCG chez des rats (Bmax) et la constante de dissociation (Kd) dependent du volume de reaction de la disposition du test. L'origine de ce phenomene est un facteur qui peut etre obtenu a partir du surnageant (105 000 g) d'un extrait testiculaire de rat et qui pourrait interferer lors de la formation du complexe hormone-recepteur. Si des corrections concernant la dilution ne sont pas faites, il en resulte par ce facteur une influence significative sur la dissociation du complexe (p < 0,001). Il est necessaire sur la base de ces observations lors d'essais de liaison de la gonadotropine de corriger l'effet de dilution par des controles adequats." @default.
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• W2078175312 title "[Interaction of gonadal receptors from rat testes with human chorionic gonadotropin: experimental implications of the dilution effect]." @default.
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