FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078219244> ?p ?o ?g. }

• W2078219244 endingPage "267" @default.
• W2078219244 startingPage "262" @default.
• W2078219244 abstract "Background Cellular adaptation to hypoxia is mediated in part by the transcription factor hypoxia-inducible factor 1 (HIF-1). Accumulating data suggest that pro-inflammatory mediators can up-regulate HIF-1α protein expression and HIF-1 DNA-binding activity in the absence of hypoxia. Accordingly, we investigated HIF-1 mediated signaling in endotoxemic rats. Materials and methods We studied three groups of male Sprague Dawley rats. Controls (N = 5) were injected i.p. with saline. Endotoxemic rats (N = 9) received a sublethal dose of lipopolysaccaride (Escherichia coli; 5 mg/kg, i.p.). A third group of rats (N = 5) received the HIF-1 stabilizing agent CoCl2 (14 mg/kg, i.p.) at T = 0 h and T = 16 h. At T = 18 h, liver microvascular perfusion was measured using laser Doppler flowmetry and hepatic tissue samples were obtained. RNA was isolated and mRNA levels of the HIF-1 dependent genes aldolase A and vascular endothelial growth factor (VEGF) were determined using quantitative real-time RT-PCR. HIF-1α content was estimated by immunoprecipitation followed by Western blotting. Results HIF-1α increased in hepatic tissue after treatment with LPS or CoCl2. LPS markedly increased hepatic expression of aldolase A, but failed to alter expression of VEGF. CoCl2 increased aldolase A and VEGF mRNA expression. Although hepatic microvascular perfusion was comparable in saline- and LPS-treated rats, hepatic microvascular blood flow and aldolase A expression were significantly inversely correlated among endotoxemic rats (r = 0.773; P = 0.003). Conclusions Increased expression of aldolase A in endotoxemic rats is mediated by both hypoxia-dependent and hypoxia-independent mechanisms. Cellular adaptation to hypoxia is mediated in part by the transcription factor hypoxia-inducible factor 1 (HIF-1). Accumulating data suggest that pro-inflammatory mediators can up-regulate HIF-1α protein expression and HIF-1 DNA-binding activity in the absence of hypoxia. Accordingly, we investigated HIF-1 mediated signaling in endotoxemic rats. We studied three groups of male Sprague Dawley rats. Controls (N = 5) were injected i.p. with saline. Endotoxemic rats (N = 9) received a sublethal dose of lipopolysaccaride (Escherichia coli; 5 mg/kg, i.p.). A third group of rats (N = 5) received the HIF-1 stabilizing agent CoCl2 (14 mg/kg, i.p.) at T = 0 h and T = 16 h. At T = 18 h, liver microvascular perfusion was measured using laser Doppler flowmetry and hepatic tissue samples were obtained. RNA was isolated and mRNA levels of the HIF-1 dependent genes aldolase A and vascular endothelial growth factor (VEGF) were determined using quantitative real-time RT-PCR. HIF-1α content was estimated by immunoprecipitation followed by Western blotting. HIF-1α increased in hepatic tissue after treatment with LPS or CoCl2. LPS markedly increased hepatic expression of aldolase A, but failed to alter expression of VEGF. CoCl2 increased aldolase A and VEGF mRNA expression. Although hepatic microvascular perfusion was comparable in saline- and LPS-treated rats, hepatic microvascular blood flow and aldolase A expression were significantly inversely correlated among endotoxemic rats (r = 0.773; P = 0.003). Increased expression of aldolase A in endotoxemic rats is mediated by both hypoxia-dependent and hypoxia-independent mechanisms." @default.
• W2078219244 created "2016-06-24" @default.
• W2078219244 creator A5001877194 @default.
• W2078219244 creator A5021248501 @default.
• W2078219244 creator A5032974614 @default.
• W2078219244 creator A5048362688 @default.
• W2078219244 creator A5052569785 @default.
• W2078219244 creator A5084193042 @default.
• W2078219244 date "2006-10-01" @default.
• W2078219244 modified "2023-09-29" @default.
• W2078219244 title "LPS Increases Hepatic HIF-1α Protein and Expression of the HIF-1-Dependent Gene Aldolase A in Rats" @default.
• W2078219244 cites W1749022239 @default.
• W2078219244 cites W1836591545 @default.
• W2078219244 cites W1973720695 @default.
• W2078219244 cites W1975644535 @default.
• W2078219244 cites W1991843895 @default.
• W2078219244 cites W2020823643 @default.
• W2078219244 cites W2021381046 @default.
• W2078219244 cites W2025974453 @default.
• W2078219244 cites W2045596016 @default.
• W2078219244 cites W2050573744 @default.
• W2078219244 cites W2062906868 @default.
• W2078219244 cites W2071179121 @default.
• W2078219244 cites W2073232859 @default.
• W2078219244 cites W2085609942 @default.
• W2078219244 cites W2101114817 @default.
• W2078219244 cites W2102526923 @default.
• W2078219244 cites W2107146684 @default.
• W2078219244 cites W2112558371 @default.
• W2078219244 cites W2130933110 @default.
• W2078219244 cites W2144625075 @default.
• W2078219244 cites W2151625241 @default.
• W2078219244 cites W4244190676 @default.
• W2078219244 cites W78888789 @default.
• W2078219244 doi "https://doi.org/10.1016/j.jss.2006.05.027" @default.
• W2078219244 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16930621" @default.
• W2078219244 hasPublicationYear "2006" @default.
• W2078219244 type Work @default.
• W2078219244 sameAs 2078219244 @default.
• W2078219244 citedByCount "18" @default.
• W2078219244 countsByYear W20782192442012 @default.
• W2078219244 countsByYear W20782192442013 @default.
• W2078219244 countsByYear W20782192442014 @default.
• W2078219244 countsByYear W20782192442015 @default.
• W2078219244 countsByYear W20782192442017 @default.
• W2078219244 countsByYear W20782192442019 @default.
• W2078219244 countsByYear W20782192442020 @default.
• W2078219244 crossrefType "journal-article" @default.
• W2078219244 hasAuthorship W2078219244A5001877194 @default.
• W2078219244 hasAuthorship W2078219244A5021248501 @default.
• W2078219244 hasAuthorship W2078219244A5032974614 @default.
• W2078219244 hasAuthorship W2078219244A5048362688 @default.
• W2078219244 hasAuthorship W2078219244A5052569785 @default.
• W2078219244 hasAuthorship W2078219244A5084193042 @default.
• W2078219244 hasConcept C104317684 @default.
• W2078219244 hasConcept C105580179 @default.
• W2078219244 hasConcept C126322002 @default.
• W2078219244 hasConcept C134018914 @default.
• W2078219244 hasConcept C150194340 @default.
• W2078219244 hasConcept C153911025 @default.
• W2078219244 hasConcept C160450060 @default.
• W2078219244 hasConcept C167734588 @default.
• W2078219244 hasConcept C178790620 @default.
• W2078219244 hasConcept C181199279 @default.
• W2078219244 hasConcept C185592680 @default.
• W2078219244 hasConcept C2777025900 @default.
• W2078219244 hasConcept C2778754761 @default.
• W2078219244 hasConcept C28165981 @default.
• W2078219244 hasConcept C3488265 @default.
• W2078219244 hasConcept C540031477 @default.
• W2078219244 hasConcept C55493867 @default.
• W2078219244 hasConcept C71924100 @default.
• W2078219244 hasConcept C7836513 @default.
• W2078219244 hasConcept C85265743 @default.
• W2078219244 hasConcept C86803240 @default.
• W2078219244 hasConceptScore W2078219244C104317684 @default.
• W2078219244 hasConceptScore W2078219244C105580179 @default.
• W2078219244 hasConceptScore W2078219244C126322002 @default.
• W2078219244 hasConceptScore W2078219244C134018914 @default.
• W2078219244 hasConceptScore W2078219244C150194340 @default.
• W2078219244 hasConceptScore W2078219244C153911025 @default.
• W2078219244 hasConceptScore W2078219244C160450060 @default.
• W2078219244 hasConceptScore W2078219244C167734588 @default.
• W2078219244 hasConceptScore W2078219244C178790620 @default.
• W2078219244 hasConceptScore W2078219244C181199279 @default.
• W2078219244 hasConceptScore W2078219244C185592680 @default.
• W2078219244 hasConceptScore W2078219244C2777025900 @default.
• W2078219244 hasConceptScore W2078219244C2778754761 @default.
• W2078219244 hasConceptScore W2078219244C28165981 @default.
• W2078219244 hasConceptScore W2078219244C3488265 @default.
• W2078219244 hasConceptScore W2078219244C540031477 @default.
• W2078219244 hasConceptScore W2078219244C55493867 @default.
• W2078219244 hasConceptScore W2078219244C71924100 @default.
• W2078219244 hasConceptScore W2078219244C7836513 @default.
• W2078219244 hasConceptScore W2078219244C85265743 @default.
• W2078219244 hasConceptScore W2078219244C86803240 @default.
• W2078219244 hasIssue "2" @default.
• W2078219244 hasLocation W20782192441 @default.

• next