Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078232238> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2078232238 endingPage "766" @default.
- W2078232238 startingPage "759" @default.
- W2078232238 abstract "In diabetes, loss of renal arteriolar smooth-muscle cell contractility leads to intraglomerular hypertension. In glomeruli isolated from streptozotocin (STZ)-induced diabetic rats, the mesangial cells (smooth muscle-like) display loss of contractile responsiveness to angiotensin II. This study examines the mechanistic relationship between altered mesangial cell contractility and vasopressor hormone-stimulated Ca2+ signaling in high glucose. Glomeruli were isolated from normal or STZ-induced diabetic rats to observe ex vivo mesangial cell contractile function. Also, rat mesangial cells were cultured (10–20 passages) in normal (5.6 mmol/1) or high (10–25.6 mmol/1) glucose for 1–5 days. Reduction of glomerular volume and decreased planar surface area of cultured mesangial cells in response to vasoconstrictor stimulation over 60 min were measured by videomicroscopy and personal computer—based morphometry. Contraction of glomeruli isolated from STZ-administered rats in response to endothelin (ET)-1 (0.1 μmol/1) or the Ca2+ ionophore A23187 (5 μmol/l) was impaired significantly compared with that in normal glucose. In the presence of arginine vasopressin (AVP) (1.0 μmol/1) or ET-1 (0.1 μmol/1), mesangial cells demonstrated a dose-dependent loss of contractile response to increasing glucose concentrations (5.6–25.6 mmol/1) within 24 h of high-glucose exposure, which was sustained for 5 days. Mesangial cells in high glucose were consistently smaller in size compared with those in normal glucose. Mesangial cells were preloaded with myo-[2-3H]inositol and intracellular [3H] inositol phosphate release in response to AVP (1.0 μmol/1) was analyzed by Dowex chromatography. Comparing cells in normal (5.6 mmol/1) versus high (25.6 mmol/1) glucose, we observed no significant difference in stimulated inositol phosphate levels from 10 to 60 s. The Ca2+-signaling response of cultured mesangial cells preloaded with Fura 2 or Indo 1 was measured by spectrofluorometry. After culture in 25.6 mmol/1 glucose, 0.1 μmol/l AVP or 0.1 μmol/1 ET-1 stimulated a cytosolic Ca2+ signal, with first and second phases, identical to that exhibited by mesangial cells in normal glucose. Fluorescence imaging of cultured mesangial cell cytoskeletal filamentous actin (F-actin) stained with rhodamine-phalloidin demonstrated reduced F-actin staining in the basal state and loss of the normal F-actin disassembly response to ET-1 in high glucose. Glomerular mesangial cells display glucose-induced altered contraction and F-actin disassembly to vasoactive stimuli, which occur in the presence of normal Ca2+ signaling." @default.
- W2078232238 created "2016-06-24" @default.
- W2078232238 creator A5016004274 @default.
- W2078232238 creator A5016293083 @default.
- W2078232238 creator A5018200032 @default.
- W2078232238 creator A5021999283 @default.
- W2078232238 creator A5025801045 @default.
- W2078232238 creator A5039062464 @default.
- W2078232238 creator A5058353865 @default.
- W2078232238 creator A5085858616 @default.
- W2078232238 date "1995-07-01" @default.
- W2078232238 modified "2023-09-27" @default.
- W2078232238 title "Glomerular Mesangial Cell Altered Contractility in High Glucose Is Ca2+ Independent" @default.
- W2078232238 doi "https://doi.org/10.2337/diab.44.7.759" @default.
- W2078232238 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7789643" @default.
- W2078232238 hasPublicationYear "1995" @default.
- W2078232238 type Work @default.
- W2078232238 sameAs 2078232238 @default.
- W2078232238 citedByCount "35" @default.
- W2078232238 countsByYear W20782322382013 @default.
- W2078232238 countsByYear W20782322382016 @default.
- W2078232238 crossrefType "journal-article" @default.
- W2078232238 hasAuthorship W2078232238A5016004274 @default.
- W2078232238 hasAuthorship W2078232238A5016293083 @default.
- W2078232238 hasAuthorship W2078232238A5018200032 @default.
- W2078232238 hasAuthorship W2078232238A5021999283 @default.
- W2078232238 hasAuthorship W2078232238A5025801045 @default.
- W2078232238 hasAuthorship W2078232238A5039062464 @default.
- W2078232238 hasAuthorship W2078232238A5058353865 @default.
- W2078232238 hasAuthorship W2078232238A5085858616 @default.
- W2078232238 hasConcept C126322002 @default.
- W2078232238 hasConcept C134018914 @default.
- W2078232238 hasConcept C163415756 @default.
- W2078232238 hasConcept C170493617 @default.
- W2078232238 hasConcept C185592680 @default.
- W2078232238 hasConcept C2776370428 @default.
- W2078232238 hasConcept C2777427919 @default.
- W2078232238 hasConcept C2778618036 @default.
- W2078232238 hasConcept C2779131090 @default.
- W2078232238 hasConcept C2779280383 @default.
- W2078232238 hasConcept C2780091579 @default.
- W2078232238 hasConcept C2908929049 @default.
- W2078232238 hasConcept C39133596 @default.
- W2078232238 hasConcept C55493867 @default.
- W2078232238 hasConcept C555293320 @default.
- W2078232238 hasConcept C71924100 @default.
- W2078232238 hasConcept C79879829 @default.
- W2078232238 hasConcept C84393581 @default.
- W2078232238 hasConcept C86803240 @default.
- W2078232238 hasConceptScore W2078232238C126322002 @default.
- W2078232238 hasConceptScore W2078232238C134018914 @default.
- W2078232238 hasConceptScore W2078232238C163415756 @default.
- W2078232238 hasConceptScore W2078232238C170493617 @default.
- W2078232238 hasConceptScore W2078232238C185592680 @default.
- W2078232238 hasConceptScore W2078232238C2776370428 @default.
- W2078232238 hasConceptScore W2078232238C2777427919 @default.
- W2078232238 hasConceptScore W2078232238C2778618036 @default.
- W2078232238 hasConceptScore W2078232238C2779131090 @default.
- W2078232238 hasConceptScore W2078232238C2779280383 @default.
- W2078232238 hasConceptScore W2078232238C2780091579 @default.
- W2078232238 hasConceptScore W2078232238C2908929049 @default.
- W2078232238 hasConceptScore W2078232238C39133596 @default.
- W2078232238 hasConceptScore W2078232238C55493867 @default.
- W2078232238 hasConceptScore W2078232238C555293320 @default.
- W2078232238 hasConceptScore W2078232238C71924100 @default.
- W2078232238 hasConceptScore W2078232238C79879829 @default.
- W2078232238 hasConceptScore W2078232238C84393581 @default.
- W2078232238 hasConceptScore W2078232238C86803240 @default.
- W2078232238 hasIssue "7" @default.
- W2078232238 hasLocation W20782322381 @default.
- W2078232238 hasLocation W20782322382 @default.
- W2078232238 hasOpenAccess W2078232238 @default.
- W2078232238 hasPrimaryLocation W20782322381 @default.
- W2078232238 hasRelatedWork W1969292293 @default.
- W2078232238 hasRelatedWork W1998631290 @default.
- W2078232238 hasRelatedWork W2005975386 @default.
- W2078232238 hasRelatedWork W2019938707 @default.
- W2078232238 hasRelatedWork W2069855804 @default.
- W2078232238 hasRelatedWork W2072872068 @default.
- W2078232238 hasRelatedWork W2092349071 @default.
- W2078232238 hasRelatedWork W2125221738 @default.
- W2078232238 hasRelatedWork W2141307244 @default.
- W2078232238 hasRelatedWork W1996764561 @default.
- W2078232238 hasVolume "44" @default.
- W2078232238 isParatext "false" @default.
- W2078232238 isRetracted "false" @default.
- W2078232238 magId "2078232238" @default.
- W2078232238 workType "article" @default.