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- W2078232467 abstract "Summary The adaptive response of the marine bacterium Sphingopyxis alaskensis RB2256 to solar radiation (both visible and ultraviolet) was assessed by a quantitative proteomic approach using iTRAQ (isobaric tags for relative and absolute quantification). Both growth phase (mid‐log and stationary phase) and duration (80 min or 8 h) of different light treatments (combinations of visible light, UV‐A and UV‐B) were assessed relative to cultures maintained in the dark. Rates of total protein synthesis and viability were also assessed. Integrating knowledge from the physiological experiments with quantitative proteomics of the 12 conditions tested provided unique insight into the adaptation biology of UV and visible light responses of S. alaskensis . High confidence identifications were obtained for 811 proteins (27% of the genome), 119 of which displayed significant quantitative differences. Mid‐log‐phase cultures produced twice as many proteomic changes as stationary‐phase cultures, while extending the duration of irradiation exposure of stationary‐phase cultures did not increase the total number of quantitative changes. Proteins with significant quantitative differences were identified that were characteristic of growth phase and light treatment, and cellular processes, pathways and interaction networks were determined. Key factors of the solar radiation adaptive response included DNA‐binding proteins implicated in reducing DNA damage, detoxification of toxic compounds such as glyoxal and reactive oxygen species, iron sequestration to minimize oxidative stress, chaperones to control protein re/folding, alterations to nitrogen metabolism, and specific changes to transcriptional and translational processes." @default.
- W2078232467 created "2016-06-24" @default.
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- W2078232467 date "2009-10-01" @default.
- W2078232467 modified "2023-10-16" @default.
- W2078232467 title "The response of the marine bacterium<i>Sphingopyxis alaskensis</i>to solar radiation assessed by quantitative proteomics" @default.
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- W2078232467 doi "https://doi.org/10.1111/j.1462-2920.2009.01992.x" @default.
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