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- W2078239174 abstract "We describe a cell-free system from HeLa cells that initiates DNA replication under cell cycle control. G1 but not G2 phase nuclei initiate replication when coincubated with S phase nuclei in cytosolic extracts from S phase but not from G1 or G2 phase HeLa cells. S phase nuclei or an S phase nuclear extract are required for the initiation of semiconservative DNA replication in G1 nuclei but not for elongation in S phase nuclei. S phase nuclear extract could be replaced by recombinant human cyclins A and E complexed to Cdk2 but not by Cdk2 alone or by human cyclin B1 complexed to Cdc2. In S phase cytosol, cyclins A/Cdk2 and E/Cdk2 triggered initiation synergistically." @default.
- W2078239174 created "2016-06-24" @default.
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- W2078239174 date "1997-01-01" @default.
- W2078239174 modified "2023-10-11" @default.
- W2078239174 title "Cyclin/Cdk-Dependent Initiation of DNA Replication in a Human Cell-Free System" @default.
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- W2078239174 doi "https://doi.org/10.1016/s0092-8674(00)81863-2" @default.
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