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• W2078239710 endingPage "690" @default.
• W2078239710 startingPage "680" @default.
• W2078239710 abstract "The fluorescence properties of the tyrosyl residues of human chorionic gonadotropin (hCG) and its α and β subunits have been examined. The effects of pH, guanidine, and disulfide cleavage on the intensity and polarization of the fluorescence suggest that the isolated subunits possess little, if any, tertiary structure beyond that which is stabilized by the disulfide bonds. Essentially all of the fluorescence of hCG and its subunits was accessible to quenching by iodide ions. Similar results were observed for several other proteins whose fluorescence originates from tyrosyl residues. Thus, we have confirmed and extended the conclusion of R. W. Cowgill ((1966) Biochim. Biophys. Acta120, 196) that the buried tyrosyl residues in ribonuclease fluoresce with a much lower quantum yield than those which are exposed. The dissociation of hCG into its subunits was accompanied by an increase in fluorescence, suggesting the exposure of tyrosyl residues. This was confirmed by difference absorption measurements which indicate a net exposure of two to three tyrosyl residues upon dissociation of the subunits. An additional 0.6 tyrosine was exposed when the disulfide bonds of the β-subunit were cleaved. The polarization of the fluorescence of hCG-β was high (P = 0.19) and, unlike several other proteins with high polarization, could not be lowered by denaturing conditions. Only by cleavage of the disulfide bonds could the fluorescence polarization of either subunit be lowered to a value (P = 0.08) characteristic of a random polypeptide. It appears that the disulfide bonds play an important role in maintaining the rigidity of the fluorescent tyrosyl residues, located at or near the surface of the protein." @default.
• W2078239710 created "2016-06-24" @default.
• W2078239710 creator A5035916458 @default.
• W2078239710 creator A5044208902 @default.
• W2078239710 creator A5086811042 @default.
• W2078239710 date "1976-04-01" @default.
• W2078239710 modified "2023-10-15" @default.
• W2078239710 title "Structural studies of human chorionic gonadotropin and its subunits using tyrosine fluorescence" @default.
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• W2078239710 doi "https://doi.org/10.1016/0003-9861(76)90306-4" @default.
• W2078239710 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5960" @default.
• W2078239710 hasPublicationYear "1976" @default.
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