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- W2078247032 abstract "Abstract A dramatic increase in the cAMP-independent protein kinase activity (10-fold) was observed in the first 10 hr of germination of excised embryonic axes of Cicer . The lag phase of enzyme induction was very short since a 6-fold stimulation of protein kinase activity was witnessed after two hr of imbibition of the axes. The increase in protein kinase activity is ascribed to de novo biosynthesis of the enzyme. Conclusive proof for the de novo biosynthesis of protein kinase was obtained by labelling the proteins in vivo with [ 35 S]-sulphate and subsequently recovering the label predominantly in the methionine residues of the purified enzyme. The purification of the enzyme to electrophoretic homogeneity (453-fold) was achieved by ion exchange chromatography followed by affinity chromatography on a Casein-Sepharose column. Polyacrylamide gel electrophoresis of the [ 35 S]-labelled enzyme revealed a single radioactive band by autoradiography, that co-electrophoresed with the Coomassie Blue stained band of protein kinase. The M , of the purified protein kinase is 94 000 as determined by molecular sieving on Sepharose CL-6B. SDS-PAGE data indicated that the enzyme is composed of two subunits of M ,s 49 000 and 62 000. Chemical characterization of the reaction product of protein kinase revealed that phosphorylation occurs at serine and threonine residues of the substrate, casein." @default.
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- W2078247032 date "1987-01-01" @default.
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- W2078247032 title "Protein kinase in Cicer embryonic axes" @default.
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- W2078247032 doi "https://doi.org/10.1016/s0031-9422(00)81720-3" @default.
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