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- W2078255054 abstract "The conventional analytical HPLC was successfully developed for micro-column separation by using a simple eluate splitting system, self-preparation of packing column and on-capillary column detector in our laboratory. Porous inlet frit in fused silica capillary was rapidly prepared by sintering stainless steel powders under 500 meshes for about 20 s. The use of such frits or metal meshes in capillary to retain C18 particles of chromatographic packing was demonstrated to be stable and specially robust with continuous packing and long chromatographic runs. Furthermore, the chromatographic behavior was detailedly evaluated by changing the flow rate and the percentage of mobile phase using the prepared capillary column. Under the optimal experimental conditions, baseline separation of the model analytes including thiourea, benzene, toluene, ethylbenzene was obtained with a high column efficiency near 70000N (plates/m) by the developed capillary-HPLC." @default.
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- W2078255054 date "2009-04-01" @default.
- W2078255054 modified "2023-10-16" @default.
- W2078255054 title "A Simple Design to Realize Micro-column Separation by Conventional Analytical HPLC" @default.
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- W2078255054 doi "https://doi.org/10.1002/cjoc.200990126" @default.
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