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• W2078265909 abstract "We report a highly effective on-chip preconcentration method by combining field-amplified sample injection (FASI) and bovine serum albumin (BSA) sweeping for ultrasensitive detection of green fluorescent protein (GFP) on a simple cross-channel microchip device. With the formation of a stagnant sample/running buffer boundary by balancing the hydrodynamic flow and the electro-osmotic flow (EOF), GFP molecules can be continuously injected into the sample loading channel and stacked. We have also demonstrated that BSA is a very effective pseudo-stationary phase for sweeping concentration of proteins in comparison to the commonly used micelles. The combination of FASI and BSA sweeping yields a concentration factor of 3570 and a limit of detection of 8.4 pM for GFP. Using this method, we have separated GFP and GFP-insulin-like growth factor-I (GFP-IGF-I) fusion protein. The entire assay (GFP concentration, matrix elimination, and electrophoretic separation) can be completed within <5 min. Furthermore, we have successfully applied this method for the detection of GFP expression of E. coli cells and the GFP content in single E. coli cells." @default.
• W2078265909 created "2016-06-24" @default.
• W2078265909 creator A5053862338 @default.
• W2078265909 creator A5056163944 @default.
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• W2078265909 date "2009-06-03" @default.
• W2078265909 modified "2023-10-16" @default.
• W2078265909 title "Combination of On-Chip Field Amplification and Bovine Serum Albumin Sweeping for Ultrasensitive Detection of Green Fluorescent Protein" @default.
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• W2078265909 doi "https://doi.org/10.1021/ac9007607" @default.
• W2078265909 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2744439" @default.
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