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• W2078271371 endingPage "4640" @default.
• W2078271371 startingPage "4633" @default.
• W2078271371 abstract "Polybrominated diphenyl ethers (PBDEs) have been shown to disrupt thyroid hormone (TH) functions on experimental animals, and one of the proposed disruption mechanisms is the competitive binding of PBDE metabolites to TH transport proteins. In this report, a nonradioactive, site-specific fluorescein–thyroxine (F–T4) conjugate was designed and synthesized as a fluorescence probe to study the binding interaction of hydroxylated PBDEs to thyroxine-binding globulin (TBG) and transthyretin (TTR), two major TH transport proteins in human plasma. Compared with free F–T4, the fluorescence intensity of TTR-bound conjugate was enhanced by as much as 2-fold, and the fluorescence polarization value of TBG-bound conjugate increased by more than 20-fold. These changes provide signal modulation mechanisms for F–T4 as a fluorescence probe. Based on fluorescence quantum yield and lifetime measurements, the fluorescence intensity enhancement was likely due to the elimination of intramolecular fluorescence quenching of fluorescein by T4 after F–T4 was bound to TTR. In circular dichroism and intrinsic tryptophan fluorescence measurements, F–T4 induced similar spectroscopic changes of the proteins as T4 did, suggesting that F–T4 bound to the proteins at the T4 binding site. By using F–T4 as the fluorescence probe in competitive binding assays, 11 OH–PBDEs with different levels of bromination and different hydroxylation positions were assessed for their binding affinity with TBG and TTR, respectively. The results indicate that the binding affinity generally increased with bromine number and OH position also played an important role. 3-OH–BDE-47 and 3′-OH–BDE-154 bound to TTR and TBG even stronger, respectively, than T4. With rising environmental level and high bioaccumulation capability, PBDEs have the potential to disrupt thyroid homeostasis by competitive binding with TH transport proteins." @default.
• W2078271371 created "2016-06-24" @default.
• W2078271371 creator A5001603792 @default.
• W2078271371 creator A5001992412 @default.
• W2078271371 date "2012-04-06" @default.
• W2078271371 modified "2023-09-30" @default.
• W2078271371 title "Assessment of the Binding of Hydroxylated Polybrominated Diphenyl Ethers to Thyroid Hormone Transport Proteins Using a Site-Specific Fluorescence Probe" @default.
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• W2078271371 doi "https://doi.org/10.1021/es2046074" @default.
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