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- W2078275995 abstract "Testosterone administration to female mice for 25 days produced a 70% increase in total kidney protein in both A/J and C57BL/6J mice. This is in contrast to the known androgen-responsive proteins, such as beta-glucuronidase and alcohol dehydrogenase, which each represent less than 1% of the total kidney proteins even after maximum stimulation. To investigate this discrepancy, we initiated a study to identify major proteins which increase with androgen treatment. Three new cytoplasmic proteins designated T1, T2, and T3 were found in different subcellular fractions of both A/J and C57BL/6J mice. T1 (43,000 daltons) and T2 (60,000 daltons) were found in the mitochondrial-lysosomal fraction of 25-day androgen-treated mice. T3 (54,000 daltons) was found in the microsomal fraction. Each of these proteins increased several-fold during androgen treatment, so that they were easily identified using sodium dodecyl sulfate polyacrylamide gel electrophoresis. By contrast, no major changes were noted in the soluble proteins. A nonhistone chromosomal protein of 54,000 mol wt (T4) was found in chromatin preparations from androgen-stimulated A/J mice. Additional studies with androgen-insensitive Tfm/Y mice and with various hormones indicated that stimulation of the T proteins was dependent on androgenic steroid and a functional androgen receptor." @default.
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- W2078275995 date "1980-04-01" @default.
- W2078275995 modified "2023-09-25" @default.
- W2078275995 title "New Androgen-Stimulated Proteins in the Kidneys of Female Mice*" @default.
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- W2078275995 doi "https://doi.org/10.1210/endo-106-4-1182" @default.
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