FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078289345> ?p ?o ?g. }

• W2078289345 endingPage "3048" @default.
• W2078289345 startingPage "3039" @default.
• W2078289345 abstract "ABSTRACT Plant pathogens cause major economic losses in the agricultural industry because late detection delays the implementation of measures that can prevent their dissemination. Sensitive and robust procedures for the rapid detection of plant pathogens are therefore required to reduce yield losses and the use of expensive, environmentally damaging chemicals. Here we describe a simple and portable system for the rapid detection of viral pathogens in infected plants based on immunofiltration, subsequent magnetic detection, and the quantification of magnetically labeled virus particles. Grapevine fanleaf virus (GFLV) was chosen as a model pathogen. Monoclonal antibodies recognizing the GFLV capsid protein were immobilized onto immunofiltration columns, and the same antibodies were linked to magnetic nanoparticles. GFLV was quantified by immunofiltration with magnetic labeling in a double-antibody sandwich configuration. A magnetic frequency mixing technique, in which a two-frequency magnetic excitation field was used to induce a sum frequency signal in the resonant detection coil, corresponding to the virus concentration within the immunofiltration column, was used for high-sensitivity quantification. We were able to measure GFLV concentrations in the range of 6 ng/ml to 20 μg/ml in less than 30 min. The magnetic immunoassay could also be adapted to detect other plant viruses, including Potato virus X and Tobacco mosaic virus , with detection limits of 2 to 60 ng/ml." @default.
• W2078289345 created "2016-06-24" @default.
• W2078289345 creator A5013094236 @default.
• W2078289345 creator A5017663416 @default.
• W2078289345 creator A5019412759 @default.
• W2078289345 creator A5036887130 @default.
• W2078289345 creator A5046068261 @default.
• W2078289345 creator A5047164999 @default.
• W2078289345 creator A5049288387 @default.
• W2078289345 creator A5071176039 @default.
• W2078289345 creator A5083258867 @default.
• W2078289345 date "2015-05-01" @default.
• W2078289345 modified "2023-09-27" @default.
• W2078289345 title "Simple and Portable Magnetic Immunoassay for Rapid Detection and Sensitive Quantification of Plant Viruses" @default.
• W2078289345 cites W128305924 @default.
• W2078289345 cites W1522996926 @default.
• W2078289345 cites W1585802206 @default.
• W2078289345 cites W1963870540 @default.
• W2078289345 cites W1966567708 @default.
• W2078289345 cites W1970079208 @default.
• W2078289345 cites W1980053683 @default.
• W2078289345 cites W1981932807 @default.
• W2078289345 cites W1990144028 @default.
• W2078289345 cites W1994052648 @default.
• W2078289345 cites W1998745175 @default.
• W2078289345 cites W2002384077 @default.
• W2078289345 cites W2012633558 @default.
• W2078289345 cites W2013537694 @default.
• W2078289345 cites W2018289835 @default.
• W2078289345 cites W2020836668 @default.
• W2078289345 cites W2028527507 @default.
• W2078289345 cites W2033729496 @default.
• W2078289345 cites W2034954866 @default.
• W2078289345 cites W2036671204 @default.
• W2078289345 cites W2038553364 @default.
• W2078289345 cites W2040466542 @default.
• W2078289345 cites W2044918208 @default.
• W2078289345 cites W2045531109 @default.
• W2078289345 cites W2046789130 @default.
• W2078289345 cites W2048404349 @default.
• W2078289345 cites W2049588389 @default.
• W2078289345 cites W2056486258 @default.
• W2078289345 cites W2057611651 @default.
• W2078289345 cites W2059595560 @default.
• W2078289345 cites W2060788264 @default.
• W2078289345 cites W2067319984 @default.
• W2078289345 cites W2067594772 @default.
• W2078289345 cites W2073879684 @default.
• W2078289345 cites W2075423460 @default.
• W2078289345 cites W2082015653 @default.
• W2078289345 cites W2083969210 @default.
• W2078289345 cites W2084855813 @default.
• W2078289345 cites W2088163512 @default.
• W2078289345 cites W2089761415 @default.
• W2078289345 cites W2090113301 @default.
• W2078289345 cites W2090848616 @default.
• W2078289345 cites W2091335921 @default.
• W2078289345 cites W2098175434 @default.
• W2078289345 cites W2100837269 @default.
• W2078289345 cites W2101108802 @default.
• W2078289345 cites W2101358020 @default.
• W2078289345 cites W2111499880 @default.
• W2078289345 cites W2126928912 @default.
• W2078289345 cites W2127223388 @default.
• W2078289345 cites W2147607919 @default.
• W2078289345 cites W2153334788 @default.
• W2078289345 cites W4230878465 @default.
• W2078289345 cites W4232937896 @default.
• W2078289345 cites W4251581896 @default.
• W2078289345 cites W87015043 @default.
• W2078289345 cites W964558111 @default.
• W2078289345 cites W1869295153 @default.
• W2078289345 doi "https://doi.org/10.1128/aem.03667-14" @default.
• W2078289345 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4393444" @default.
• W2078289345 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25710366" @default.
• W2078289345 hasPublicationYear "2015" @default.
• W2078289345 type Work @default.
• W2078289345 sameAs 2078289345 @default.
• W2078289345 citedByCount "43" @default.
• W2078289345 countsByYear W20782893452017 @default.
• W2078289345 countsByYear W20782893452018 @default.
• W2078289345 countsByYear W20782893452019 @default.
• W2078289345 countsByYear W20782893452020 @default.
• W2078289345 countsByYear W20782893452021 @default.
• W2078289345 countsByYear W20782893452022 @default.
• W2078289345 countsByYear W20782893452023 @default.
• W2078289345 crossrefType "journal-article" @default.
• W2078289345 hasAuthorship W2078289345A5013094236 @default.
• W2078289345 hasAuthorship W2078289345A5017663416 @default.
• W2078289345 hasAuthorship W2078289345A5019412759 @default.
• W2078289345 hasAuthorship W2078289345A5036887130 @default.
• W2078289345 hasAuthorship W2078289345A5046068261 @default.
• W2078289345 hasAuthorship W2078289345A5047164999 @default.
• W2078289345 hasAuthorship W2078289345A5049288387 @default.
• W2078289345 hasAuthorship W2078289345A5071176039 @default.
• W2078289345 hasAuthorship W2078289345A5083258867 @default.
• W2078289345 hasBestOaLocation W20782893451 @default.
• W2078289345 hasConcept C109110057 @default.

• next